The marine intertidal zone shapes oyster and clam digestive bacterial microbiota

• Published in: FEMS Microbiology Ecology
• Main Authors: Offret, Clement, Paulino, Sauvann, Gauthier, Olivier, Chateau, Kevin, Bidault, Adeline, Corporeau, Charlotte, Miner, Philippe, Petton, Bruno, Pernet, Fabrice, Fabioux, Caroline, Paillard, Christine, Le Blay, Gwenaelle
• Other Authors: Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Institut Français de Recherche pour l'Exploitation de la Mer - Brest (IFREMER Centre de Bretagne), ANR-17-EURE-0015,ISBlue,Interdisciplinary Graduate School for the Blue planet(2017), European Project: 678589,H2020,H2020-SFS-2015-2,VIVALDI(2016)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2020
• Subjects: ACL; pacific oysters; climate-change; stress; crassostrea-gigas; diversity; digestive gland; clams; oysters; communities; microbiota; sydney rock oysters; ruditapes-decussatus; depuration; gut microflora; intertidal zone; metabarcoding; envir; geo; Pacific; Crassostrea gigas
• Online Access: https://doi.org/10.1093/femsec/fiaa078; https://hal.archives-ouvertes.fr/hal-03004625

WOS:000574373300003 International audience Digestive microbiota provide a wide range of beneficial effects on host physiology and are therefore likely to play a key role in marine intertidal bivalve ability to acclimatize to the intertidal zone. This study investigated the effect of intertidal levels on the digestive bacterial microbiota of oysters (Crassostrea gigas) and clams (Ruditapes philippinarum), two bivalves with different ecological niches. Based on 16S rRNA region sequencing, digestive glands, seawater and sediments harbored specific bacterial communities, dominated by operational taxonomic units assigned to the Mycoplasmatales, Desulfobacterales and Rhodobacterales orders, respectively. Field implantation modified digestive bacterial microbiota of both bivalve species according to their intertidal position. Rhodospirillales and Legionellales abundances increased in oysters and clams from the low intertidal level, respectively. After a 14-day depuration process, these effects were still observed, especially for clams, while digestive bacterial microbiota of oysters were subjected to more short-term environmental changes. Nevertheless, 3.5 months stay on an intertidal zone was enough to leave an environmental footprint on the digestive bacterial microbiota, suggesting the existence of autochthonous bivalve bacteria. When comparing clams from the three intertidal levels, 20% of the bacterial assemblage was shared among the levels and it was dominated by an operational taxonomic unit affiliated to the Mycoplasmataceae and Spirochaetaceae families.