Protective effect of four potential probiotics against pathogen-challenge of the larvae of three bivalves: Pacific oyster (Crassostrea gigas), flat oyster (Ostrea edulis) and scallop (Pecten maximus)

• Published in: Aquaculture
• Main Authors: Kesarcodi-Watson, A., Miner, Philippe, Nicolas, Jean-Louis, Robert, René
• Other Authors: Laboratoire de Physiologie des Invertébrés Plouzané (LPI), Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER), Laboratoire des Sciences de l'Environnement Marin (LEMAR) (LEMAR), Institut de Recherche pour le Développement (IRD)-Institut Français de Recherche pour l'Exploitation de la Mer (IFREMER)-Université de Brest (UBO)-Institut Universitaire Européen de la Mer (IUEM), Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Brest (UBO)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Physiologie des Invertébrés (LPI)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2012
• Subjects: Probiotic; Flat oyster; Mollusc; Scallop; Pacific oyster; envir; socio; Pacific; Crassostrea gigas
• Online Access: https://doi.org/10.1016/j.aquaculture.2012.02.029; https://hal.univ-brest.fr/hal-00788483

International audience Mollusc aquaculture is an important commodity for France with Pacific oysters, flat oysters and the great scallop being the three main species. Pacific oyster larvae production is currently successful, however larval production of flat oyster and scallops regularly face problems remedied by antibiotics. Using a bioassay technique, four potential probiotic strains (Alteromonas macleodii 0444, Neptunomonas sp. 0536, Phaeobacter gallaeciensis, Pseudoaltermonas sp. D41) were tested upon the larvae of the three mollusc species when challenged with pathogens. Pacific oysters and flat oysters were challenged individually with the pathogens Vibrio coralliilyticus and V. pectenicida, while scallops were challenged with V. coralliilyticus and V. splendidus. Survival of the larvae was measured in challenged larvae given the probiotics and those without probiotic. In scallop larvae, protection against V. coralliilyticus and V. splendidus was provided by prior administration of P. gallaeciensis (29% and 53% better than the respective pathogen controls), A. macleodii 0444 (30%, 36%) and Neptunomonas sp. 0536 (36%, 55%). Significant protection with Pseudoalteromonas sp. D41 was only provided against V. splendidus (35%). In flat oyster larvae, protection against V. coralliilyticus and V. pectenicida was provided by P. gallaeciensis. (73%, 69%) and A. macleodii 0444 (45%, 53%). Neptunomonas sp. 0536 provided significant protection only against V. pectenicida (48% higher). Use of Pseudoalteromonas sp. D41 did not offer significant protection for flat oyster larvae against either pathogen. Experiments with Pacific oysters suggested that Pseudoalteromonas sp. D41 and P. gallaeciensis could be effective against challenge by V. coralliilyticus (50% and 40% improved survival respectively) but not against V. pectenicida. The results indicate that P. gallaeciensis, A. macleodii 0444, Neptunomonas sp. 0536 and Pseudoalteromonas sp. D41 could be useful in mollusc larviculture.