Quantitative determination of marine lipophilic toxins in mussels, oysters and cockles using liquid chromatography ? mass spectrometry. Interlaboratory validation study

• Published in: Food Additives & Contaminants: Part A
• Language: English
• Published: Taylor & Francis 2012
• Subjects: Life Sciences; Canada; Crassostrea gigas
• Online Access: http://hdl.handle.net/2262/65429; https://doi.org/10.1080/19440049.2011.608382

Abstract Thirteen laboratories participated in an interlaboratory study to evaluate the method performance characteristics of a liquid chromatography tandem mass spectrometric method (LC-MS/MS) for marine lipophilic shellfish toxins. Method performance characteristics were evaluated for mussel (Mytilus edulis), oyster (Crassostrea gigas) and cockle (Cerastoderma edule) matrices. The specific toxin analogues tested included okadaic acid (OA), dinophysistoxins-1 and -2 (DTX1,-2), azaspiracids-1, -2 and -3 (AZA1,-2,-3), pectenotoxin-2 (PTX2), yessotoxin (YTX) and 45-OH-yessotoxin (45-OH-YTX). The instrumental technique was developed as an alternative to the still widely applied biological methods (mouse or rat bioassay). Validation was done according to the AOAC harmonised protocol for the design, conduct and interpretation of method-performance studies. Eight different test materials were sent as blind duplicates to the participating laboratories. Twelve laboratories returned results that were accepted to be included in the statistical evaluation. The method precision was expressed as HORRATs. For the individual toxins (except for 45-OH-YTX) HORRATs were found to be ? 1.8 (median HORRAT: 0.8) in all tested materials. The recoveries of OA-, AZA- and YTX- group toxins were within the range of 80 ? 108% and for PTX2 was within the range of 62 ? 93%. Based on the acceptable values for precision and recovery, it was concluded that the method is suitable for official control purposes to quantitatively determine OA/DTXs, AZAs, YTXs and PTX2 in shellfish. : van Egmond, Hans, [e-mail] merged with this user on 17-Feb-2011 by Gilbert, John; van Egmond, Hans, [e-mail] merged with this user on 06-May-2008 by Haire, Lynsey (van Egmond, Hans) [e-mail] (van den Top, Hester J.) [e-mail] (Gerssen, Arjen) [e-mail] (McCarron, Pearse) [e-mail] (van Egmond, Hans) RIKILT Institute of Food Safety, Wageningen UR - Wageningen - NETHERLANDS (van den Top, Hester J.) RIKILT Institute of Food Safety, Wageningen UR - Wageningen - NETHERLANDS (Gerssen, Arjen) National Research Council, Institute for Marine Biosciences - 1411 Oxford Street--> - B3H3Z1 - Halifax - CANADA (McCarron, Pearse) RIKILT Institute of Food Safety, Wageningen UR, - Wageningen - NETHERLANDS (van Egmond, Hans) CANADA NETHERLANDS