Water-in-ionic liquid microemulsion-based organogels as novel matrices for enzyme immobilization
Abstract The use of water-in-ionic liquid microemulsion-based organogels (w/IL MBGs) as novel supports for the immobilization of lipase B from Candida antarctica and lipase from Chromobacterium viscosum was investigated. These novel lipase-containing w/IL MBGs can be effectively used as solid phase...
| Published in: | Biotechnology Journal |
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| Language: | English |
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Wiley-Blackwell
2011
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| Online Access: | http://hdl.handle.net/2262/48385 https://doi.org/10.1002/biot.201000052 |
| Summary: | Abstract The use of water-in-ionic liquid microemulsion-based organogels (w/IL MBGs) as novel supports for the immobilization of lipase B from Candida antarctica and lipase from Chromobacterium viscosum was investigated. These novel lipase-containing w/IL MBGs can be effectively used as solid phase biocatalysts in various polar and non-polar organic solvents or ionic liquids, exhibiting up to 4.4-fold higher esterification activity compared to water-in-oil microemulsion-based organogels. The immobilized lipases retain their activity for several hours at 70 o C, while their half life time is up to 25-fold higher compared to that observed in w/IL microemulsions. Fourier transform infrared spectroscopy data indicate that immobilized lipases adopt a more rigid structure, referring to the structure in aqueous solution, which is in correlation with their enhanced catalytic behaviour observed. ipavlidi@cc.uoi.gr (Pavlidis, Ioannis V) tzafker@hotmail.com (Tzafestas, Kyriakos) hstamati@cc.uoi.gr (Stamatis, Haralambos) University of Ioannina, Biological Applications and Technologies - GREECE (Pavlidis, Ioannis V) University of Ioannina, Biological Applications and Technologies - GREECE (Tzafestas, Kyriakos) University of Ioannina, Biological Applications and Technologies - GREECE (Stamatis, Haralambos) GREECE |
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