Development of a Polymerase Chain Reaction Assay for Specific Identification of Clostridium colinum

• Published in: Avian Pathology
• Language: English
• Published: Taylor & Francis 2010
• Subjects: Life Sciences; Macklin; Iceland
• Online Access: http://hdl.handle.net/2262/46687; https://doi.org/10.1080/03079450801918662

Abstract Clostridium colinum is the causative agent of ulcerative enteritis (UE), a serious disease of bobwhite quail (Coluinus virginianus) and sporadically of young chickens. The aim of the present study was to develop a polymerase chain reaction assay specific for C. colinum identification. The 16S rDNA sequence of C. colinum was analyzed and two species-specific primers were designed. The specificity of these primers was tested with closely related Clostridium species and the expected amplified product (935 bp) was observed only with DNA from samples containing C. colinum. Results from performing PCR assays on fecal samples from quails spiked with different concentrations of C. colinum showed that the detection limit of the assay was 1.6 x 104 CFU per gram of fecal material. This PCR assay can be used in diagnostic laboratories to confirm the presence of C. colinum in pure cultures and could be used to screen enriched samples or fecal samples for the presence of this pathogen. [e-mail] (Bano, Luca) [e-mail] (Drigo, Ilenia) [e-mail] (Macklin, Kenneth S.) [e-mail] (Oyarzabal, Omar A.) [e-mail] (Bilgili, Sacit F.) Istituto Zooprofilattico delle Venezie - viale Brigata Treviso 13/a--> - 31100 - Treviso - ITALY (Bano, Luca) ICELAND (Bano, Luca) Istituto Zooprofilattico delle Venezie - Treviso - ITALY (Drigo, Ilenia) Auburn University, Poultry Science - Auburn - UNITED STATES (Macklin, Kenneth S.) Auburn University, Poultry Science - Auburn - UNITED STATES (Oyarzabal, Omar A.) Auburn University, Poultry Science - Auburn - UNITED STATES (Bilgili, Sacit F.) ICELAND ITALY UNITED STATES