Compound specific isotope analyses of harp seal teeth : tools for trophic ecology reconstruction

• Published in: Marine Ecology Progress Series
• Main Authors: Kershaw, Joanna Louise, de la Vega, Camille, Jeffreys, Rachel, Frie, Anne Kristine, Haug, Tore, Mahaffey, Claire, Mettam, Colin, Stenson, Garry, Smout, Sophie Caroline
• Other Authors: NERC, University of St Andrews. School of Biology, University of St Andrews. Sea Mammal Research Unit, University of St Andrews. Scottish Oceans Institute, University of St Andrews. Centre for Research into Ecological & Environmental Modelling, University of St Andrews. Coastal Resources Management Group
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2022
• Subjects: Phocid seals; Foraging specialisation; Isotopic niche; Trophic position; Diet; Dentine; Inert tissues; QH301 Biology; Aquatic Science; Ecology; Evolution; Behavior and Systematics; NDAS; NERC; SDG 14 - Life Below Water; MCC; QH301; Arctic; Arctic Ocean; Harp Seal; Northwest Atlantic; Pagophilus groenlandicus
• Online Access: http://hdl.handle.net/10023/26370; https://doi.org/10.3354/meps13867

Funding: This work resulted from the ARISE project (NE/P006035/1 awarded to CM and RJ, and NE/P00623X/1 awarded to SS), part of the Changing Arctic Ocean programme, funded by the UKRI Natural Environment Research Council (NERC). As sentinels of ecosystem health, high trophic level predators integrate information through all levels of the food web. Their tissues can be used to investigate spatiotemporal variability in foraging behaviour, and with the appropriate analytical methods and tools, archived samples can be used to reconstruct past trophic interactions. Harp seal (Pagophilus groenlandicus) teeth collected in the 1990s from the Northwest Atlantic were analysed for bulk stable carbon and nitrogen isotopes (δ13Cbulk and δ15Nbulk), and compound specific stable nitrogen isotopes of amino acids (δ15NAA) for the first time. We developed a fine-scale, annual growth layer group (GLG) dentine sub-sampling method corresponding to their second and third year of life. In accordance with previous diet studies, while there was individual variability in δ15Nbulk, δ13Cbulk, and δ15NAA measurements, we did not detect significant differences in isotopic niche widths between males and females, or between GLGs. Relative trophic position was calculated as the baseline corrected δ15NAA values using trophic (glutamic acid) and source (phenylalanine and glycine) amino acids. Variability was measured between individuals in their relative trophic position, but within individual variability was low, suggesting that they fed at the same trophic level over these 2 years of life. These novel δ15NAA data may therefore suggest individual, specialist harp seal foraging behaviour in sub-adults. Our findings show that compound specific stable isotope signatures of archived, inert predator tissues can be used as tools for the retrospective reconstruction of trophic interactions on broad spatiotemporal scales. Postprint Peer reviewed