Synthesis of next generation maleimide radical labels

This work was funded by EPSRC, Grant Number EP/LO22044/1, a Royal Society University Research Fellowship to JEL , Royal Society Research Grant RG120645 and support from Active Spectrum Inc. The synthesis and characterization of four new nitroxide-radical-containing next-generation maleimides are pre...

Full description

Bibliographic Details
Published in:Synlett
Main Authors: Hajjaj, Bouchra, Shah, Anokhi, Bell, Stacey, Shirran, Sally Lorna, Botting, Catherine Helen, Slawin, Alexandra Martha Zoya, Hulme, Alison, Lovett, Janet Eleanor
Other Authors: EPSRC, The Royal Society, University of St Andrews. School of Physics and Astronomy, University of St Andrews. School of Biology, University of St Andrews. School of Chemistry, University of St Andrews. EaSTCHEM, University of St Andrews. Biomedical Sciences Research Complex
Format: Article in Journal/Newspaper
Language:English
Published: 2017
Subjects:
Next-generation maleimide
Succinimide
Nitroxide spin label
Radical
EPR spectroscopy
QC Physics
QD Chemistry
DAS
QC
QD
Sperm whale
Online Access:http://hdl.handle.net/10023/11153
https://doi.org/10.1055/s-0035-1562451
Description
Summary:This work was funded by EPSRC, Grant Number EP/LO22044/1, a Royal Society University Research Fellowship to JEL , Royal Society Research Grant RG120645 and support from Active Spectrum Inc. The synthesis and characterization of four new nitroxide-radical-containing next-generation maleimides are presented. Each new label has a single leaving group which is either a phenoxyl or bromide. The linker between the maleimide and the nitroxide-containing framework is either a racemic mixture of a short chain or an achiral longer chain. These molecules have been designed to site-specifically label vicinal cysteines in proteins for magnetic resonance studies. The characterization of the final products includes crystallography and the labeling of sperm whale myoglobin protein. Postprint Peer reviewed

Similar Items