Eel Kisspeptins: Identification, Functional Activity, and Inhibition on both Pituitary LH and GnRH Receptor Expression
The European eel (Anguilla anguilla) presents a blockade of sexual maturation at a prepubertal stage due to a deficient production of gonadotropins. We previously initiated, in the eel, the investigation of the kisspeptin system, one of the major gatekeepers of puberty in mammals, and we predicted t...
Published in: | Frontiers in Endocrinology |
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Main Authors: | , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
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Frontiers media sa
2018
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Online Access: | http://hdl.handle.net/10668/19217 https://doi.org/10.3389/fendo.2017.00353 https://www.frontiersin.org/articles/10.3389/fendo.2017.00353/pdf |
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ftsspa:oai:www.repositoriosalud.es:10668/19217 |
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record_format |
openpolar |
institution |
Open Polar |
collection |
Sistema Sanitario Público de Andalucía (SSPA): Repositorio |
op_collection_id |
ftsspa |
language |
English |
topic |
kisspeptins tissue distribution functional activity pituitary cell culture LH GnRH receptor European eel Metastasis-suppressor gene Protein-coupled receptor Gonadotropin-releasing-hormone European sea bass In-vivo Molecular characterization Luteinizing-hormone Anguilla-anguilla Scombroid fish Chub mackerel |
spellingShingle |
kisspeptins tissue distribution functional activity pituitary cell culture LH GnRH receptor European eel Metastasis-suppressor gene Protein-coupled receptor Gonadotropin-releasing-hormone European sea bass In-vivo Molecular characterization Luteinizing-hormone Anguilla-anguilla Scombroid fish Chub mackerel Pasquier, Jeremy Lafont, Anne-Gaelle Denis, Florian Lefranc, Benjamin Dubessy, Christophe Moreno-Herrera, Antonio Vaudry, Hubert Leprince, Jerome Dufour, Sylvie Rousseau, Karine Eel Kisspeptins: Identification, Functional Activity, and Inhibition on both Pituitary LH and GnRH Receptor Expression |
topic_facet |
kisspeptins tissue distribution functional activity pituitary cell culture LH GnRH receptor European eel Metastasis-suppressor gene Protein-coupled receptor Gonadotropin-releasing-hormone European sea bass In-vivo Molecular characterization Luteinizing-hormone Anguilla-anguilla Scombroid fish Chub mackerel |
description |
The European eel (Anguilla anguilla) presents a blockade of sexual maturation at a prepubertal stage due to a deficient production of gonadotropins. We previously initiated, in the eel, the investigation of the kisspeptin system, one of the major gatekeepers of puberty in mammals, and we predicted the sequence of two Kiss genes. In the present study, we cloned and sequenced Kiss1 and Kiss2 cDNAs from the eel brain. The tissue distributions of Kiss1 and Kiss2 transcripts, as investigated by quantitative real-time PCR, showed that both genes are primarily expressed in the eel brain and pituitary. The two 10-residue long sequences characteristic of kisspeptin, eel Kp1(10) and Kp2(10), as well as two longer sequences, predicted as mature peptides, eel Kp1(15) and Kp2(12), were synthesized and functionally analyzed. Using rat Kiss1 receptor-transfected Chinese hamster ovary cells, we found that the four synthesized eel peptides were able to induce [Ca2+](i) responses, indicating their ability to bind mammalian KissR-1 and to activate second messenger pathways. In primary culture of eel pituitary cells, all four peptides were able to specifically and dose-dependently inhibit lh beta expression, without any effect on fsh beta, confirming our previous data with heterologous kisspeptins. Furthermore, in this eel in vitro system, all four peptides inhibited the expression of the type 2 GnRH receptor (gnrh-r2). Our data revealed a dual inhibitory effect of homologous kisspeptins on both pituitary lh beta and gnrh-r2 expression in the European eel. |
author2 |
Pasquier, Jeremy UPMC, Museum Natl Hist Nat, Res Unit BOREA Biol Aquat Organisms & Ecosyst, CNRS 7208,IRD207,UCN,UA, Paris, France Lafont, Anne-Gaelle UPMC, Museum Natl Hist Nat, Res Unit BOREA Biol Aquat Organisms & Ecosyst, CNRS 7208,IRD207,UCN,UA, Paris, France Denis, Florian UPMC, Museum Natl Hist Nat, Res Unit BOREA Biol Aquat Organisms & Ecosyst, CNRS 7208,IRD207,UCN,UA, Paris, France Dufour, Sylvie UPMC, Museum Natl Hist Nat, Res Unit BOREA Biol Aquat Organisms & Ecosyst, CNRS 7208,IRD207,UCN,UA, Paris, France Rousseau, Karine UPMC, Museum Natl Hist Nat, Res Unit BOREA Biol Aquat Organisms & Ecosyst, CNRS 7208,IRD207,UCN,UA, Paris, France Lefranc, Benjamin Normandy Univ, Lab Neuronal & Neuroendocrine Differentiat & Comm, INSERM, U1239, Rouen, France Dubessy, Christophe Normandy Univ, Lab Neuronal & Neuroendocrine Differentiat & Comm, INSERM, U1239, Rouen, France Moreno-Herrera, Antonio Normandy Univ, Lab Neuronal & Neuroendocrine Differentiat & Comm, INSERM, U1239, Rouen, France Vaudry, Hubert Normandy Univ, Lab Neuronal & Neuroendocrine Differentiat & Comm, INSERM, U1239, Rouen, France Leprince, Jerome Normandy Univ, Lab Neuronal & Neuroendocrine Differentiat & Comm, INSERM, U1239, Rouen, France Moreno-Herrera, Antonio Univ Cordoba, Reina Sofia Univ Hosp, Maimonides Inst Biomed Res Cordoba IMIBIC, Dept Cell Biol Physiol & Immunol, Cordoba, Spain European Community PRO-EEL National Research Agency NEMO FrenchKiSS |
format |
Article in Journal/Newspaper |
author |
Pasquier, Jeremy Lafont, Anne-Gaelle Denis, Florian Lefranc, Benjamin Dubessy, Christophe Moreno-Herrera, Antonio Vaudry, Hubert Leprince, Jerome Dufour, Sylvie Rousseau, Karine |
author_facet |
Pasquier, Jeremy Lafont, Anne-Gaelle Denis, Florian Lefranc, Benjamin Dubessy, Christophe Moreno-Herrera, Antonio Vaudry, Hubert Leprince, Jerome Dufour, Sylvie Rousseau, Karine |
author_sort |
Pasquier, Jeremy |
title |
Eel Kisspeptins: Identification, Functional Activity, and Inhibition on both Pituitary LH and GnRH Receptor Expression |
title_short |
Eel Kisspeptins: Identification, Functional Activity, and Inhibition on both Pituitary LH and GnRH Receptor Expression |
title_full |
Eel Kisspeptins: Identification, Functional Activity, and Inhibition on both Pituitary LH and GnRH Receptor Expression |
title_fullStr |
Eel Kisspeptins: Identification, Functional Activity, and Inhibition on both Pituitary LH and GnRH Receptor Expression |
title_full_unstemmed |
Eel Kisspeptins: Identification, Functional Activity, and Inhibition on both Pituitary LH and GnRH Receptor Expression |
title_sort |
eel kisspeptins: identification, functional activity, and inhibition on both pituitary lh and gnrh receptor expression |
publisher |
Frontiers media sa |
publishDate |
2018 |
url |
http://hdl.handle.net/10668/19217 https://doi.org/10.3389/fendo.2017.00353 https://www.frontiersin.org/articles/10.3389/fendo.2017.00353/pdf |
genre |
Anguilla anguilla |
genre_facet |
Anguilla anguilla |
op_relation |
1664-2392 http://hdl.handle.net/10668/19217 doi:10.3389/fendo.2017.00353 https://www.frontiersin.org/articles/10.3389/fendo.2017.00353/pdf 419461600001 |
op_rights |
Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ open access |
op_doi |
https://doi.org/10.3389/fendo.2017.00353 |
container_title |
Frontiers in Endocrinology |
container_volume |
8 |
_version_ |
1782331111557300224 |
spelling |
ftsspa:oai:www.repositoriosalud.es:10668/19217 2023-11-12T04:01:12+01:00 Eel Kisspeptins: Identification, Functional Activity, and Inhibition on both Pituitary LH and GnRH Receptor Expression Pasquier, Jeremy Lafont, Anne-Gaelle Denis, Florian Lefranc, Benjamin Dubessy, Christophe Moreno-Herrera, Antonio Vaudry, Hubert Leprince, Jerome Dufour, Sylvie Rousseau, Karine Pasquier, Jeremy UPMC, Museum Natl Hist Nat, Res Unit BOREA Biol Aquat Organisms & Ecosyst, CNRS 7208,IRD207,UCN,UA, Paris, France Lafont, Anne-Gaelle UPMC, Museum Natl Hist Nat, Res Unit BOREA Biol Aquat Organisms & Ecosyst, CNRS 7208,IRD207,UCN,UA, Paris, France Denis, Florian UPMC, Museum Natl Hist Nat, Res Unit BOREA Biol Aquat Organisms & Ecosyst, CNRS 7208,IRD207,UCN,UA, Paris, France Dufour, Sylvie UPMC, Museum Natl Hist Nat, Res Unit BOREA Biol Aquat Organisms & Ecosyst, CNRS 7208,IRD207,UCN,UA, Paris, France Rousseau, Karine UPMC, Museum Natl Hist Nat, Res Unit BOREA Biol Aquat Organisms & Ecosyst, CNRS 7208,IRD207,UCN,UA, Paris, France Lefranc, Benjamin Normandy Univ, Lab Neuronal & Neuroendocrine Differentiat & Comm, INSERM, U1239, Rouen, France Dubessy, Christophe Normandy Univ, Lab Neuronal & Neuroendocrine Differentiat & Comm, INSERM, U1239, Rouen, France Moreno-Herrera, Antonio Normandy Univ, Lab Neuronal & Neuroendocrine Differentiat & Comm, INSERM, U1239, Rouen, France Vaudry, Hubert Normandy Univ, Lab Neuronal & Neuroendocrine Differentiat & Comm, INSERM, U1239, Rouen, France Leprince, Jerome Normandy Univ, Lab Neuronal & Neuroendocrine Differentiat & Comm, INSERM, U1239, Rouen, France Moreno-Herrera, Antonio Univ Cordoba, Reina Sofia Univ Hosp, Maimonides Inst Biomed Res Cordoba IMIBIC, Dept Cell Biol Physiol & Immunol, Cordoba, Spain European Community PRO-EEL National Research Agency NEMO FrenchKiSS 2018-01-08 http://hdl.handle.net/10668/19217 https://doi.org/10.3389/fendo.2017.00353 https://www.frontiersin.org/articles/10.3389/fendo.2017.00353/pdf en eng Frontiers media sa 1664-2392 http://hdl.handle.net/10668/19217 doi:10.3389/fendo.2017.00353 https://www.frontiersin.org/articles/10.3389/fendo.2017.00353/pdf 419461600001 Attribution 4.0 International http://creativecommons.org/licenses/by/4.0/ open access kisspeptins tissue distribution functional activity pituitary cell culture LH GnRH receptor European eel Metastasis-suppressor gene Protein-coupled receptor Gonadotropin-releasing-hormone European sea bass In-vivo Molecular characterization Luteinizing-hormone Anguilla-anguilla Scombroid fish Chub mackerel research article VoR 2018 ftsspa https://doi.org/10.3389/fendo.2017.00353 2023-10-29T17:30:42Z The European eel (Anguilla anguilla) presents a blockade of sexual maturation at a prepubertal stage due to a deficient production of gonadotropins. We previously initiated, in the eel, the investigation of the kisspeptin system, one of the major gatekeepers of puberty in mammals, and we predicted the sequence of two Kiss genes. In the present study, we cloned and sequenced Kiss1 and Kiss2 cDNAs from the eel brain. The tissue distributions of Kiss1 and Kiss2 transcripts, as investigated by quantitative real-time PCR, showed that both genes are primarily expressed in the eel brain and pituitary. The two 10-residue long sequences characteristic of kisspeptin, eel Kp1(10) and Kp2(10), as well as two longer sequences, predicted as mature peptides, eel Kp1(15) and Kp2(12), were synthesized and functionally analyzed. Using rat Kiss1 receptor-transfected Chinese hamster ovary cells, we found that the four synthesized eel peptides were able to induce [Ca2+](i) responses, indicating their ability to bind mammalian KissR-1 and to activate second messenger pathways. In primary culture of eel pituitary cells, all four peptides were able to specifically and dose-dependently inhibit lh beta expression, without any effect on fsh beta, confirming our previous data with heterologous kisspeptins. Furthermore, in this eel in vitro system, all four peptides inhibited the expression of the type 2 GnRH receptor (gnrh-r2). Our data revealed a dual inhibitory effect of homologous kisspeptins on both pituitary lh beta and gnrh-r2 expression in the European eel. Article in Journal/Newspaper Anguilla anguilla Sistema Sanitario Público de Andalucía (SSPA): Repositorio Frontiers in Endocrinology 8 |