Eel Kisspeptins: Identification, Functional Activity, and Inhibition on both Pituitary LH and GnRH Receptor Expression

• Published in: Frontiers in Endocrinology
• Main Authors: Pasquier, Jeremy, Lafont, Anne-Gaelle, Denis, Florian, Lefranc, Benjamin, Dubessy, Christophe, Moreno-Herrera, Antonio, Vaudry, Hubert, Leprince, Jerome, Dufour, Sylvie, Rousseau, Karine
• Other Authors: Pasquier, Jeremy UPMC, Museum Natl Hist Nat, Res Unit BOREA Biol Aquat Organisms & Ecosyst, CNRS 7208,IRD207,UCN,UA, Paris, France, Lafont, Anne-Gaelle UPMC, Museum Natl Hist Nat, Res Unit BOREA Biol Aquat Organisms & Ecosyst, CNRS 7208,IRD207,UCN,UA, Paris, France, Denis, Florian UPMC, Museum Natl Hist Nat, Res Unit BOREA Biol Aquat Organisms & Ecosyst, CNRS 7208,IRD207,UCN,UA, Paris, France, Dufour, Sylvie UPMC, Museum Natl Hist Nat, Res Unit BOREA Biol Aquat Organisms & Ecosyst, CNRS 7208,IRD207,UCN,UA, Paris, France, Rousseau, Karine UPMC, Museum Natl Hist Nat, Res Unit BOREA Biol Aquat Organisms & Ecosyst, CNRS 7208,IRD207,UCN,UA, Paris, France, Lefranc, Benjamin Normandy Univ, Lab Neuronal & Neuroendocrine Differentiat & Comm, INSERM, U1239, Rouen, France, Dubessy, Christophe Normandy Univ, Lab Neuronal & Neuroendocrine Differentiat & Comm, INSERM, U1239, Rouen, France, Moreno-Herrera, Antonio Normandy Univ, Lab Neuronal & Neuroendocrine Differentiat & Comm, INSERM, U1239, Rouen, France, Vaudry, Hubert Normandy Univ, Lab Neuronal & Neuroendocrine Differentiat & Comm, INSERM, U1239, Rouen, France, Leprince, Jerome Normandy Univ, Lab Neuronal & Neuroendocrine Differentiat & Comm, INSERM, U1239, Rouen, France, Moreno-Herrera, Antonio Univ Cordoba, Reina Sofia Univ Hosp, Maimonides Inst Biomed Res Cordoba IMIBIC, Dept Cell Biol Physiol & Immunol, Cordoba, Spain, European Community PRO-EEL, National Research Agency NEMO, FrenchKiSS
• Format: Article in Journal/Newspaper
• Language: English
• Published: Frontiers media sa 2018
• Subjects: kisspeptins; tissue distribution; functional activity; pituitary cell culture; LH; GnRH receptor; European eel; Metastasis-suppressor gene; Protein-coupled receptor; Gonadotropin-releasing-hormone; European sea bass; In-vivo; Molecular characterization; Luteinizing-hormone; Anguilla-anguilla; Scombroid fish; Chub mackerel; Anguilla anguilla
• Online Access: http://hdl.handle.net/10668/19217; https://doi.org/10.3389/fendo.2017.00353; https://www.frontiersin.org/articles/10.3389/fendo.2017.00353/pdf

The European eel (Anguilla anguilla) presents a blockade of sexual maturation at a prepubertal stage due to a deficient production of gonadotropins. We previously initiated, in the eel, the investigation of the kisspeptin system, one of the major gatekeepers of puberty in mammals, and we predicted the sequence of two Kiss genes. In the present study, we cloned and sequenced Kiss1 and Kiss2 cDNAs from the eel brain. The tissue distributions of Kiss1 and Kiss2 transcripts, as investigated by quantitative real-time PCR, showed that both genes are primarily expressed in the eel brain and pituitary. The two 10-residue long sequences characteristic of kisspeptin, eel Kp1(10) and Kp2(10), as well as two longer sequences, predicted as mature peptides, eel Kp1(15) and Kp2(12), were synthesized and functionally analyzed. Using rat Kiss1 receptor-transfected Chinese hamster ovary cells, we found that the four synthesized eel peptides were able to induce [Ca2+](i) responses, indicating their ability to bind mammalian KissR-1 and to activate second messenger pathways. In primary culture of eel pituitary cells, all four peptides were able to specifically and dose-dependently inhibit lh beta expression, without any effect on fsh beta, confirming our previous data with heterologous kisspeptins. Furthermore, in this eel in vitro system, all four peptides inhibited the expression of the type 2 GnRH receptor (gnrh-r2). Our data revealed a dual inhibitory effect of homologous kisspeptins on both pituitary lh beta and gnrh-r2 expression in the European eel.