Deciphering mollusc shell production: the roles of genetic mechanisms through to ecology, aquaculture and biomimetics

• Published in: Biological Reviews
• Main Authors: Clark, Melody S, Peck, Lloyd S, Arivalagan, Jaison, Backeljau, Thierry, Berland, Sophie, Cardoso, Joao C R, Caurcel, Carlos, Chapelle, Gauthier, De Noia, Michele, Dupont, Sam, Gharbi, Karim, Hoffman, Joseph I, Last, Kim S, Marie, Arul, Melzner, Frank, Michalek, Kati, Morris, James, Power, Deborah M, Ramesh, Kirti, Sanders, Trystan, Sillanpää, Kirsikka, Sleight, Victoria A, Stewart-Sinclair, Phoebe J, Sundell, Kristina, Telesca, Luca, Vendrami, David L J, Ventura, Alexander, Wilding, Thomas A, Yarra, Tejaswi, Harper, Elizabeth M
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2020
• Subjects: New Zealand; Antarc*; Antarctica
• Online Access: https://eprints.soton.ac.uk/448577/; https://eprints.soton.ac.uk/448577/1/brv.12640.pdf

Most molluscs possess shells, constructed from a vast array of microstructures and architectures. The fully formed shell is composed of calcite or aragonite. These CaCO3 crystals form complex biocomposites with proteins, which although typically less than 5% of total shell mass, play significant roles in determining shell microstructure. Despite much research effort, large knowledge gaps remain in how molluscs construct and maintain their shells, and how they produce such a great diversity of forms. Here we synthesize results on how shell shape, microstructure, composition and organic content vary among, and within, species in response to numerous biotic and abiotic factors. At the local level, temperature, food supply and predation cues significantly affect shell morphology, whilst salinity has a much stronger influence across latitudes. Moreover, we emphasize how advances in genomic technologies [e.g. restriction site-associated DNA sequencing (RAD-Seq) and epigenetics] allow detailed examinations of whether morphological changes result from phenotypic plasticity or genetic adaptation, or a combination of these. RAD-Seq has already identified single nucleotide polymorphisms associated with temperature and aquaculture practices, whilst epigenetic processes have been shown significantly to modify shell construction to local conditions in, for example, Antarctica and New Zealand. We also synthesize results on the costs of shell construction and explore how these affect energetic trade-offs in animal metabolism. The cellular costs are still debated, with CaCO3 precipitation estimates ranging from 1-2 J/mg to 17-55 J/mg depending on experimental and environmental conditions. However, organic components are more expensive (~29 J/mg) and recent data indicate transmembrane calcium ion transporters can involve considerable costs. This review emphasizes the role that molecular analyses have played in demonstrating multiple evolutionary origins of biomineralization genes. Although these are characterized by ...