| Summary: | NGSfilter file.The ngsfilter file containing the sample - PCR tag information necessary for demultiplexing the sequence data in OBITools.140704_SND104_B_L001_GZO-13.ngsfilterPost OBITools analysis scriptThe R script contains the various commands that were used for filtering the metabarcoding data and matching it to the vegetation.FilterDNAandCompareToVegetation.RVegetation dataThe results of the vegetation surveys of the 11 lakes. Abundance assignment in the >2m survey is follows: Dominant taxa are recorded as 4, common taxa as 3, scattered taxa as 2 and rare taxa as 1.Vegetation.csvMetabarcoding resultsThe OBITools output, expanded with the lake name, core number, depth information and and a shorter taxa code used for linking the results to the vegetation data.IdentifiedDNA.tsvSample code informationFile that links the sample codes used in the study to the lake name, core and depth information.SampleToSampleInfo.tsvForward reads (part 1)Compressed FASTQ file containing the first half of the forward reads.140704_SND104_B_L001_GZO-13_R1-1.fastq.gzForward reads (part 2)Compressed FASTQ file containing the second half of the forward reads.140704_SND104_B_L001_GZO-13_R1-2.fastq.gzReverse reads (part 1)Compressed FASTQ file containing the first half of the reverse reads.140704_SND104_B_L001_GZO-13_R2-1.fastq.gzReverse reads (part 2)Compressed FASTQ file containing the second half of the reverse reads.140704_SND104_B_L001_GZO-13_R2-2.fastq.gz,Metabarcoding of lake sediments have been shown to reveal current and past biodiversity, but little is known about the degree to which taxa growing in the vegetation are represented in environmental DNA (eDNA) records. We analysed composition of lake and catchment vegetation and vascular plant eDNA at 11 lakes in northern Norway. Out of 489 records of taxa growing within 2 m from the lake shore, 17-49% (mean 31%) of the identifiable taxa recorded were detected with eDNA. Of the 217 eDNA records of 47 plant taxa in the 11 lakes, 73% and 12% matched taxa recorded in ...
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