The carbohydrate moiety of serum IgM from Atlantic cod (Gadus morhua L.)

• Published in: Fish & Shellfish Immunology
• Main Authors: Magnadóttir, Bergljót, Crispin, Max, Royle, Louise, Colominas, Cristina, Harvey, David J., Dwek, Raymond A., Rudd, Pauline M.
• Format: Article in Journal/Newspaper
• Language: English
• Published: 2002
• Subjects: atlantic cod; Gadus morhua
• Online Access: https://eprints.soton.ac.uk/414688/

The carbohydrate moiety of cod serum IgM was analysed using oligosaccharide sequencing techniques. The carbohydrate moiety constituted about 10% of the molecular weight of cod IgM, was associated with the constant region of the heavy chains (Fc), and was composed of N-linked complex type oligosaccharides. Considerable heterogeneity was observed. Sixteen different glycan structures were identified, over 60% were sialylated and 40% contained core fucose. The carbohydrate moiety of cod IgM was shown to provide protection against protease digestion, and partial deglycosylation abolished the antigen binding property of natural cod anti-TNP-BSA antibody.