The carbohydrate moiety of serum IgM from Atlantic cod (Gadus morhua L.)

The carbohydrate moiety of cod serum IgM was analysed using oligosaccharide sequencing techniques. The carbohydrate moiety constituted about 10% of the molecular weight of cod IgM, was associated with the constant region of the heavy chains (Fc), and was composed of N-linked complex type oligosaccha...

Full description

Bibliographic Details
Published in:Fish & Shellfish Immunology
Main Authors: Magnadóttir, Bergljót, Crispin, Max, Royle, Louise, Colominas, Cristina, Harvey, David J., Dwek, Raymond A., Rudd, Pauline M.
Format: Article in Journal/Newspaper
Language:English
Published: 2002
Subjects:
atlantic cod
Gadus morhua
Online Access:https://eprints.soton.ac.uk/414688/
Description
Summary:The carbohydrate moiety of cod serum IgM was analysed using oligosaccharide sequencing techniques. The carbohydrate moiety constituted about 10% of the molecular weight of cod IgM, was associated with the constant region of the heavy chains (Fc), and was composed of N-linked complex type oligosaccharides. Considerable heterogeneity was observed. Sixteen different glycan structures were identified, over 60% were sialylated and 40% contained core fucose. The carbohydrate moiety of cod IgM was shown to provide protection against protease digestion, and partial deglycosylation abolished the antigen binding property of natural cod anti-TNP-BSA antibody.

Similar Items