Temperature sensitivity of bat antibodies links metabolic state of bats with antigen-recognition diversity
We would like to express our gratitude to the volunteers who assisted us in the field: Boris Petrov, Petar Velkov, Katrin Dimitrova, Kaloyana Koseva, Kristin Meshinska, and Stela-Teodora Trendafilova. Our heartfelt thanks go to Dr Lubka Roumenina, and Dr Sebastien Lacroix-Desmazes (both from Centre...
Published in: | Nature Communications |
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Main Authors: | , , , , , , , , , , |
Other Authors: | , , , , , , , , , , , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
HAL CCSD
2024
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Subjects: | |
Online Access: | https://pasteur.hal.science/pasteur-04675437 https://pasteur.hal.science/pasteur-04675437v1/document https://pasteur.hal.science/pasteur-04675437v1/file/Temperature%20sensitivity%20of%20bat%20antibodies%20links%20metabolic%20state%20of%20bats%20with%20antigen-recognition%20diversity%20-%2041467_2024_Article_50316.pdf https://doi.org/10.1038/s41467-024-50316-x |
Summary: | We would like to express our gratitude to the volunteers who assisted us in the field: Boris Petrov, Petar Velkov, Katrin Dimitrova, Kaloyana Koseva, Kristin Meshinska, and Stela-Teodora Trendafilova. Our heartfelt thanks go to Dr Lubka Roumenina, and Dr Sebastien Lacroix-Desmazes (both from Centre de Recherche des Cordeliers, Paris, France) for their insightful feedback on our ideas and their unwavering intellectual support throughout this study. We thank Dr Hugo Mouquet, Dr Vishu Kumar Aimanianda (both from Institute Pasteur, Paris, France) and Dr Stephane Mesnage, (University of Sheffield, UK) for providing us with some important material (pathogen proteins, antibodies, and plasmids). We also thank Pr. J. Pikula and Dr. V. Kovácová (Department of Ecology and Diseases of Zoo Animals, Game, Fish and Bees, University of Veterinary and Pharmaceutical Sciences Brno) for sharing the MmNep cells. International audience The bat immune system features multiple unique properties such as dampened inflammatory responses and increased tissue protection, explaining their long lifespan and tolerance to viral infections. Here, we demonstrated that body temperature fluctuations corresponding to different physiological states in bats exert a large impact on their antibody repertoires. At elevated temperatures typical for flight, IgG from the bat species Myotis myotis and Nyctalus noctula show elevated antigen binding strength and diversity, recognizing both pathogen-derived antigens and autoantigens. The opposite is observed at temperatures reflecting inactive physiological states. IgG antibodies of human and other mammals, or antibodies of birds do not appear to behave in a similar way. Importantly, diversification of bat antibody specificities results in preferential recognition of damaged endothelial and epithelial cells, indicating an anti-inflammatory function. The temperature-sensitivity of bat antibodies is mediated by the variable regions of immunoglobulin molecules. Additionally, we uncover specific molecular ... |
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