First evidence for a direct inhibitory effect of kisspeptins on LH expression in the eel, Anguilla anguilla

• Published in: General and Comparative Endocrinology
• Main Authors: Pasquier, Jérémy, Lafont, Anne-Gaëlle, Leprince, Jérôme, Vaudry, Hubert, Rousseau, Karine, Dufour, Sylvie
• Other Authors: Biologie des Organismes et Ecosystèmes Aquatiques (BOREA), Université de Caen Normandie (UNICAEN), Normandie Université (NU)-Normandie Université (NU)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Différenciation et communication neuronale et neuroendocrine (DC2N), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Fédératif de Recherches Multidisciplinaires sur les Peptides (IFRMP 23), CHU Rouen, Normandie Université (NU)-Normandie Université (NU)-Université Le Havre Normandie (ULH), Normandie Université (NU)-Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre de Lutte Contre le Cancer Henri Becquerel Normandie Rouen (CLCC Henri Becquerel)-Centre National de la Recherche Scientifique (CNRS), ANR-08-BLAN-0173,Puberteel,Neuroendocrine control of eel puberty.(2008), ANR-07-BLAN-0056,FrenchKiSS,French REsearch Network for the CHaracterization of KiSS-1/GPR54 in the neuroendocrine control of reproduction: potential therapeutic applications(2007)
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2011
• Subjects: MESH: Amino Acid Sequence; MESH: Anguilla; MESH: Cells; Cultured; MESH: Female; MESH: Gonadotropins; MESH: Kisspeptins; MESH: Luteinizing Hormone; MESH: Molecular Sequence Data; MESH: Phylogeny; MESH: Pituitary Gland; MESH: Real-Time Polymerase Chain Reaction; MESH: Receptors; G-Protein-Coupled; MESH: Reverse Transcriptase Polymerase Chain Reaction; MESH: Sequence Alignment; MESH: Animals; MESH: Base Sequence; [CHIM.THER]Chemical Sciences/Medicinal Chemistry; [SDV.BC.IC]Life Sciences [q-bio]/Cellular Biology/Cell Behavior [q-bio.CB]; [SDV.NEU.NB]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC]/Neurobiology; [SDV.SP.PHARMA]Life Sciences [q-bio]/Pharmaceutical sciences/Pharmacology; Anguilla anguilla
• Online Access: https://normandie-univ.hal.science/hal-01973832; https://doi.org/10.1016/j.ygcen.2011.05.019

International audience The kisspeptin system has emerged as one of the main puberty gatekeepers among vertebrates. The European eel (Anguilla anguilla) is a remarkable model due to its phylogenetical position at the basis of teleosts, and its unique life cycle with a blockade of puberty before reproductive migration. We cloned the full-length coding sequence of a kisspeptin receptor (Kissr) in the eel. Comparison of Kissr sequences assigned the eel Kissr to a basal position in a clade including most of the known teleost Kissr, in agreement with the eel phylogenetical position. Eel Kissr tissue distribution was analyzed by quantitative real-time PCR. Eel Kissr was highly expressed in the brain, especially in the telencephalon and di-/mes-encephalon, while a very low or undetectable expression was observed in various peripheral organs. A high expression of Kissr was also found in the pituitary indicating a possible direct pituitary role of kisspeptin. Primary cultures of eel pituitary cells were performed to investigate the direct effects of kisspeptin on pituitary hormone expression. Human/lamprey kisspeptin exerted a time- and dose-dependent inhibitory effect on LHβ expression. All other tested kisspeptins had a similar inhibitory effect on LHβ expression. The inhibitory effect of kisspeptins was exerted specifically on LHβ as no change was induced on the expression of other glycoprotein hormone subunits (GPα, FSHβ and TSHβ) nor of growth hormone. These data provide the first evidence for the existence, in the European eel, of a kisspeptin system, which may play a direct inhibitory role on pituitary LHβ expression.