Purification and molecular weight determination of WT SpTrx and SpTR.

(A) SDS-polyacrylamide gel electrophoresis of WT SpTrx and SpTR after protein purification. (B) Determination of the molecular weight of native SpTrx and SpTR by size-exclusion chromatography on a Superdex 200 10/300 GL column. Thyroglobulin (667 kDa), γ-globulin (150 kDa), albumin (44.3 kDa), and r...

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Bibliographic Details
Main Authors: Thu-Thuy Nguyen (9325402), Trang Hoang (676675), Kiet N. Tran (9099305), Hyeonji Kim (3839122), Sei-Heon Jang (2828522), ChangWoo Lee (2828525)
Format: Still Image
Language:unknown
Published: 2021
Subjects:
Biophysics
Biochemistry
Molecular Biology
Biotechnology
Ecology
Environmental Sciences not elsewhere classified
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
small redox protein
psychrophilic arctic bacterium
high temperatures regardless
f26a mutants resulted
div >< p
common structure consisting
aromatic amino acids
f26 mutants compared
exhibits thermal stability
aromatic residues
aromatic cluster
β2 terminus
study examined
studied extensively
structural stability
structural instability
sphingomonas </
spectroscopic investigations
site conformation
sheet flanked
results suggest
pamc 26621
induced unfolding
including psychrophiles
fully elucidated
f26 showed
f26 changed
central β
buried phenylalanine
buried inside
buried f26
aliphatic cluster
adapted organisms
Arctic
Online Access:https://doi.org/10.1371/journal.pone.0261123.g002
Description
Summary:(A) SDS-polyacrylamide gel electrophoresis of WT SpTrx and SpTR after protein purification. (B) Determination of the molecular weight of native SpTrx and SpTR by size-exclusion chromatography on a Superdex 200 10/300 GL column. Thyroglobulin (667 kDa), γ-globulin (150 kDa), albumin (44.3 kDa), and ribonuclease A (13.7 kDa).

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