Additional file 5 of Revealing the composition of the eukaryotic microbiome of oyster spat by CRISPR-Cas Selective Amplicon Sequencing (CCSAS)

Additional file 5: Figure S2. Eukaryotic taxa representing >1% of the sequences revealed by deep-sequencing of the 18S rRNA amplicons for oyster spat samples, using "universal" 18S primers (Table S3), non-metazoan primers (Table S3), blocking primers (Table S3), or CRISPR-Cas Selective...

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Main Authors: Kevin Xu Zhong (5769752), Anna Cho (3217569), Christoph M. Deeg (6786839), Amy M. Chan (11758124), Curtis A. Suttle (11758127)
Format: Other Non-Article Part of Journal/Newspaper
Language:unknown
Published: 2021
Subjects:
Microbiology
Genetics
Eukaryotic microbiome
18S rRNA gene
Microeukaryote
CRISPR-Cas
Taxon-specific single-guide RNA
gRNA target site
CasOligo
CCSAS
Pacific
Pacific oyster
Online Access:https://doi.org/10.6084/m9.figshare.17084303.v1
id ftsmithonian:oai:figshare.com:article/17084303
record_format openpolar
spelling ftsmithonian:oai:figshare.com:article/17084303 2023-05-15T17:54:17+02:00 Additional file 5 of Revealing the composition of the eukaryotic microbiome of oyster spat by CRISPR-Cas Selective Amplicon Sequencing (CCSAS) Kevin Xu Zhong (5769752) Anna Cho (3217569) Christoph M. Deeg (6786839) Amy M. Chan (11758124) Curtis A. Suttle (11758127) 2021-11-26T05:00:00Z https://doi.org/10.6084/m9.figshare.17084303.v1 unknown https://figshare.com/articles/journal_contribution/Additional_file_5_of_Revealing_the_composition_of_the_eukaryotic_microbiome_of_oyster_spat_by_CRISPR-Cas_Selective_Amplicon_Sequencing_CCSAS_/17084303 doi:10.6084/m9.figshare.17084303.v1 CC BY + CC0 CC0 CC-BY Microbiology Genetics Eukaryotic microbiome 18S rRNA gene Microeukaryote CRISPR-Cas Taxon-specific single-guide RNA gRNA target site CasOligo CCSAS Text Journal contribution 2021 ftsmithonian https://doi.org/10.6084/m9.figshare.17084303.v1 2021-12-19T20:46:01Z Additional file 5: Figure S2. Eukaryotic taxa representing >1% of the sequences revealed by deep-sequencing of the 18S rRNA amplicons for oyster spat samples, using "universal" 18S primers (Table S3), non-metazoan primers (Table S3), blocking primers (Table S3), or CRISPR-Cas Selective Amplicon Sequencing (CCSAS) combining "universal" 18S primers and CRISPR-Cas9 with pacific-oyster-specific sgRNA m258 (Table S2). Other Non-Article Part of Journal/Newspaper Pacific oyster Unknown Pacific
institution Open Polar
collection Unknown
op_collection_id ftsmithonian
language unknown
topic Microbiology
Genetics
Eukaryotic microbiome
18S rRNA gene
Microeukaryote
CRISPR-Cas
Taxon-specific single-guide RNA
gRNA target site
CasOligo
CCSAS
spellingShingle Microbiology
Genetics
Eukaryotic microbiome
18S rRNA gene
Microeukaryote
CRISPR-Cas
Taxon-specific single-guide RNA
gRNA target site
CasOligo
CCSAS
Kevin Xu Zhong (5769752)
Anna Cho (3217569)
Christoph M. Deeg (6786839)
Amy M. Chan (11758124)
Curtis A. Suttle (11758127)
Additional file 5 of Revealing the composition of the eukaryotic microbiome of oyster spat by CRISPR-Cas Selective Amplicon Sequencing (CCSAS)
topic_facet Microbiology
Genetics
Eukaryotic microbiome
18S rRNA gene
Microeukaryote
CRISPR-Cas
Taxon-specific single-guide RNA
gRNA target site
CasOligo
CCSAS
description Additional file 5: Figure S2. Eukaryotic taxa representing >1% of the sequences revealed by deep-sequencing of the 18S rRNA amplicons for oyster spat samples, using "universal" 18S primers (Table S3), non-metazoan primers (Table S3), blocking primers (Table S3), or CRISPR-Cas Selective Amplicon Sequencing (CCSAS) combining "universal" 18S primers and CRISPR-Cas9 with pacific-oyster-specific sgRNA m258 (Table S2).
format Other Non-Article Part of Journal/Newspaper
author Kevin Xu Zhong (5769752)
Anna Cho (3217569)
Christoph M. Deeg (6786839)
Amy M. Chan (11758124)
Curtis A. Suttle (11758127)
author_facet Kevin Xu Zhong (5769752)
Anna Cho (3217569)
Christoph M. Deeg (6786839)
Amy M. Chan (11758124)
Curtis A. Suttle (11758127)
author_sort Kevin Xu Zhong (5769752)
title Additional file 5 of Revealing the composition of the eukaryotic microbiome of oyster spat by CRISPR-Cas Selective Amplicon Sequencing (CCSAS)
title_short Additional file 5 of Revealing the composition of the eukaryotic microbiome of oyster spat by CRISPR-Cas Selective Amplicon Sequencing (CCSAS)
title_full Additional file 5 of Revealing the composition of the eukaryotic microbiome of oyster spat by CRISPR-Cas Selective Amplicon Sequencing (CCSAS)
title_fullStr Additional file 5 of Revealing the composition of the eukaryotic microbiome of oyster spat by CRISPR-Cas Selective Amplicon Sequencing (CCSAS)
title_full_unstemmed Additional file 5 of Revealing the composition of the eukaryotic microbiome of oyster spat by CRISPR-Cas Selective Amplicon Sequencing (CCSAS)
title_sort additional file 5 of revealing the composition of the eukaryotic microbiome of oyster spat by crispr-cas selective amplicon sequencing (ccsas)
publishDate 2021
url https://doi.org/10.6084/m9.figshare.17084303.v1
geographic Pacific
geographic_facet Pacific
genre Pacific oyster
genre_facet Pacific oyster
op_relation https://figshare.com/articles/journal_contribution/Additional_file_5_of_Revealing_the_composition_of_the_eukaryotic_microbiome_of_oyster_spat_by_CRISPR-Cas_Selective_Amplicon_Sequencing_CCSAS_/17084303
doi:10.6084/m9.figshare.17084303.v1
op_rights CC BY + CC0
op_rightsnorm CC0
CC-BY
op_doi https://doi.org/10.6084/m9.figshare.17084303.v1
_version_ 1766162030453063680

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