Additional file 5 of Revealing the composition of the eukaryotic microbiome of oyster spat by CRISPR-Cas Selective Amplicon Sequencing (CCSAS)

Additional file 5: Figure S2. Eukaryotic taxa representing >1% of the sequences revealed by deep-sequencing of the 18S rRNA amplicons for oyster spat samples, using "universal" 18S primers (Table S3), non-metazoan primers (Table S3), blocking primers (Table S3), or CRISPR-Cas Selective...

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Bibliographic Details
Main Authors: Kevin Xu Zhong (5769752), Anna Cho (3217569), Christoph M. Deeg (6786839), Amy M. Chan (11758124), Curtis A. Suttle (11758127)
Format: Other Non-Article Part of Journal/Newspaper
Language:unknown
Published: 2021
Subjects:
Microbiology
Genetics
Eukaryotic microbiome
18S rRNA gene
Microeukaryote
CRISPR-Cas
Taxon-specific single-guide RNA
gRNA target site
CasOligo
CCSAS
Pacific
Pacific oyster
Online Access:https://doi.org/10.6084/m9.figshare.17084303.v1
Description
Summary:Additional file 5: Figure S2. Eukaryotic taxa representing >1% of the sequences revealed by deep-sequencing of the 18S rRNA amplicons for oyster spat samples, using "universal" 18S primers (Table S3), non-metazoan primers (Table S3), blocking primers (Table S3), or CRISPR-Cas Selective Amplicon Sequencing (CCSAS) combining "universal" 18S primers and CRISPR-Cas9 with pacific-oyster-specific sgRNA m258 (Table S2).

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