Genotyping error rates among software packages using a minimum read depth of 16.

• Main Authors: Isabel Salado (11604107), Alberto Fernández-Gil (578149), Carles Vilà (197062), Jennifer A. Leonard (9071318)
• Format: Dataset
• Language: unknown
• Published: 2021
• Subjects: Genetics; Molecular Biology; Evolutionary Biology; Ecology; Biological Sciences not elsewhere classified; single multiplex reaction; reliable individual identification; relatively high number; new bioinformatic tools; low quality samples; iberian wolf (<; canis lupus </; software packages infer; recent software developments; four software packages; high quality genotype; ngs data may; generation sequencing data; div >< p; genotype microsatellite loci; 19 loci analyzed; automated microsatellite genotyping; microsatellite loci; generation sequencing; automated genotyping; genotyping process; time consuming; smaller amount; sequence reads; reasonable cost; provided genotypes; population characterization; pcr replicates; multiplexed reactions; laboratory work; kinship determination; increasing throughput; highly unoptimized; highly polymorphic; genotypes directly; generally short; first time; final analyses; fewer errors; fecal dna; endangered species; 10 replicates; Canis lupus
• Online Access: https://doi.org/10.1371/journal.pone.0258906.t003

Proportion genotyped , the number of genotypes estimated by each software ( N genotypes ) divided by the total number of reference genotypes per locus across all replicates and samples. Genotyping success , the number of genotypes that coincide with the reference ( N successful ) divided by the total number of genotypes estimated by the software per locus across all replicates and samples ( N genotypes ). Proportion ADO , the number of heterozygote genotypes for which only one of the two alleles could be genotyped ( N ADO ) divided by the number of heterozygote genotypes in the reference. Proportion FA , the number of genotypes including a false allele ( N FA ) divided by the total number of reference genotypes. Values are shown as mean ± standard error per locus.