Relation between genotyping success and number of replicates using Megasat.

• Main Authors: Isabel Salado (11604107), Alberto Fernández-Gil (578149), Carles Vilà (197062), Jennifer A. Leonard (9071318)
• Format: Still Image
• Language: unknown
• Published: 2021
• Subjects: Genetics; Molecular Biology; Evolutionary Biology; Ecology; Biological Sciences not elsewhere classified; single multiplex reaction; reliable individual identification; relatively high number; new bioinformatic tools; low quality samples; iberian wolf (<; canis lupus </; software packages infer; recent software developments; four software packages; high quality genotype; ngs data may; generation sequencing data; div >< p; genotype microsatellite loci; 19 loci analyzed; automated microsatellite genotyping; microsatellite loci; generation sequencing; automated genotyping; genotyping process; time consuming; smaller amount; sequence reads; reasonable cost; provided genotypes; population characterization; pcr replicates; multiplexed reactions; laboratory work; kinship determination; increasing throughput; highly unoptimized; highly polymorphic; genotypes directly; generally short; first time; final analyses; fewer errors; fecal dna; endangered species; 10 replicates; Canis lupus
• Online Access: https://doi.org/10.1371/journal.pone.0258906.g003

Single locus (a): although the probability of genotyping success is higher for homozygotes (0.66; dashed line) than for heterozygotes (0.46; solid line), seven independent replicates are required to determine a homozygous genotype in a noninvasive sample, while eight were for a heterozygote. Multiple loci (b, c): Probability of obtaining the correct genotype for multiple homozygous (b, dashed lines) and heterozygous (c, solid lines) loci with different number of replicates. Considering a total of 19 loci, probabilities were calculated to obtain a correct genotype for at least 16 (squares), 17 (circles), 18 (triangles) and 19 (diamonds) of the 19 loci. Horizontal line marks probability of 0.95.