Artificial induction of sleep restores memory impairment in Aβ 42 Arctic expressing flies.

• Main Authors: Jenifer C. Kaldun (11530225), Shahnaz R. Lone (11530228), Ana M. Humbert Camps (11530231), Cornelia Fritsch (436027), Yves F. Widmer (11530234), Jens V. Stein (3154206), Seth M. Tomchik (9155250), Simon G. Sprecher (10058941)
• Format: Still Image
• Language: unknown
• Published: 2021
• Subjects: Cell Biology; Molecular Biology; Neuroscience; Mental Health; Biological Sciences not elsewhere classified; involved circuitry ameliorates; div >< p; >< sub ><; &# 946 ;</; modulate forgetting caused; regulating neuronal excitability; molecular disease pathways; drosophila melanogaster </; drosophila </; accelerates forgetting; neuronal expression; arctic </; 42 </; well understood; targeted expression; similar effects; related dementia; promising mechanism; pathological hallmark; olfactory memories; mushroom bodies; memory defects; main causes; fly brain; feeding levetiracetam; central component; alzheimer disease; ></ sup; Arctic
• Online Access: https://doi.org/10.1371/journal.pbio.3001412.g004

(B, D) Flies were transferred to 29°C after appetitive conditioning to induce sleep by activating dorsal fan-shaped body neurons with dTrpA1 . (A, B) dTrpA1 was expressed with 104y-Gal4 . (A) Performance indices of the 3 tested genotypes were not significantly different from each other immediately after conditioning (n ≥ 7). (B) Inducing sleep for 23 h after conditioning could restore 24-h memory in flies, which express Aβ 42 Arctic in the MB (n ≥ 14). (C, D) Sleep was induced by using R23E10-Gal4 . (C) Directly after conditioning, no significant difference between the tested genotypes was observed (n ≥ 12). (D) 24 h after training, inducing sleep was able to rescue the memory defect of flies expressing Aβ 42 Arctic in the MB (n ≥ 11). (E, F) The drug Gabadoxol (THIP) was used to induce sleep in AD flies. Feeding THIP enhanced LTM tested 24 h after aversive spaced training in flies expressing Aβ 42 Arctic in the brain (E; n ≥ 8) or specifically in the MB (F; n ≥ 8). See S5 Fig for the effect of THIP on sleep and S1 Table for the data. All other details are similar to Fig 1 . AD, Alzheimer disease; LTM, long-term memory; MB, mushroom body.