| Summary: | (B, D) Flies were transferred to 29°C after appetitive conditioning to induce sleep by activating dorsal fan-shaped body neurons with dTrpA1 . (A, B) dTrpA1 was expressed with 104y-Gal4 . (A) Performance indices of the 3 tested genotypes were not significantly different from each other immediately after conditioning (n ≥ 7). (B) Inducing sleep for 23 h after conditioning could restore 24-h memory in flies, which express Aβ 42 Arctic in the MB (n ≥ 14). (C, D) Sleep was induced by using R23E10-Gal4 . (C) Directly after conditioning, no significant difference between the tested genotypes was observed (n ≥ 12). (D) 24 h after training, inducing sleep was able to rescue the memory defect of flies expressing Aβ 42 Arctic in the MB (n ≥ 11). (E, F) The drug Gabadoxol (THIP) was used to induce sleep in AD flies. Feeding THIP enhanced LTM tested 24 h after aversive spaced training in flies expressing Aβ 42 Arctic in the brain (E; n ≥ 8) or specifically in the MB (F; n ≥ 8). See S5 Fig for the effect of THIP on sleep and S1 Table for the data. All other details are similar to Fig 1 . AD, Alzheimer disease; LTM, long-term memory; MB, mushroom body.
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