Spectral composition of the experimental light.

• Main Authors: Mariann Eilertsen (11502317), Benjamin G. J. Clokie (11502320), Lars O. E. Ebbesson (2622343), Cristina Tanase (11502323), Herve Migaud (308426), Jon Vidar Helvik (8028887)
• Format: Still Image
• Language: unknown
• Published: 2021
• Subjects: Biophysics; Cell Biology; Molecular Biology; Neuroscience; Physiology; Evolutionary Biology; Immunology; Developmental Biology; Science Policy; Mental Health; vertebrate ancient opsin; major receptive organs; emitting diodes technology; div >< p; putative opsin stimulation; potential direct activation; lateral tuberal nuclus; adapted salmon parr; stimulated neuronal activity; salmo salar </; photoreceptors expressing melanopsin; light sensory organs; melanopsin expressing cells; specific brain regions; similar brain regions; photosensitive brain regions; atlantic salmon (<; following light stimulation; deep brain photoreceptors; brain neural activity; activated following dark; neuronal modulation activity; overlapping expression patterns; neural activity; lateral cells; deep brain; atlantic salmon; light stimulation; neural activation; nonvisual photoreceptors; neuronal signals; melanopsin expression; teleost brain; specifically activated; salmon exposed; situ </; fos </; red light; visual world; various parts; suprachiasmatic nucleus; study aimed; strongest signal; pineal organ; pineal innervations; photoreceptive inputs; known presence; known marker; fish exposed; expression increased; either independent; different wavelengths; different spectra; apparent difference; 120 minutes; Atlantic salmon; Salmo salar
• Online Access: https://doi.org/10.1371/journal.pone.0258007.g001

LED units (Signify) generated narrow bandwidth lights of Blue (444 nm peak), Green (523 nm peak) or Red (632 nm peak). Broad-spectrum white light was delivered using a wide spectrum compact fluorescent bulb (Viva-Lite). Intensity in each tank for all light treatments was calibrated to 5 W/m 2 .