Binding of IAVs to biotinylated SGPs.

• Main Authors: Johanna West (11473573), Juliane Röder (3241677), Tatyana Matrosovich (11473576), Jana Beicht (11473579), Jan Baumann (3526571), Nancy Mounogou Kouassi (8745879), Jennifer Doedt (6818249), Nicolai Bovin (371933), Gianpiero Zamperin (7912553), Michele Gastaldelli (7832834), Annalisa Salviato (6880550), Francesco Bonfante (4163383), Sergei Kosakovsky Pond (9238695), Sander Herfst (327462), Ron Fouchier (8708322), Jochen Wilhelm (353946), Hans-Dieter Klenk (39881), Mikhail Matrosovich (253583)
• Format: Still Image
• Language: unknown
• Published: 2021
• Subjects: Biochemistry; Cell Biology; Genetics; Molecular Biology; Evolutionary Biology; Infectious Diseases; Virology; Biological Sciences not elsewhere classified; studied recombinant variants; results advance understanding; iav ha required; ha sequences revealed; type amino acids; inferred avian precursor; xlink ">; substitutions r62i; substitutions q226l; receptor specificity; phenotypic effects; known effects; human adaptation; hong kong; corresponding positions; 1968 differed; Ruddy Turnstone
• Online Access: https://doi.org/10.1371/journal.ppat.1009566.g003

The solid-phase adsorbed viruses were allowed to bind biotinylated SGPs from solution, and association constants of viral complexes with SGPs (K ass ) were determined as described in Materials and Methods. Higher values of K ass reflect stronger binding. ( a) Binding to six low molecular mass Neu5Acα2-3Gal-containing SGPs differing by structure of penultimate sugar moieties. Wild type IAVs A/mallard/Alberta/279/1998 (H3N8) and A/ruddy turnstone/ Delaware/2378/1988 (H7N7) were tested in parallel with R7 and R2. Two to 4 experiments were performed on different days with one replicate for each virus-SGP pair per experiment. ( b) Binding to high molecular mass SGPs 3’SLN and 6’SLN. Two experiments were performed on different days with two replicates per each virus-SGP pair per experiment. All panels show the individual values adjusted for day as described in Materials and Methods with geometric mean (bars) and SDs. P values for the differences between Kass presented in the panel 3a are shown in S3 Table .