| Summary: | The solid-phase adsorbed viruses were allowed to bind biotinylated SGPs from solution, and association constants of viral complexes with SGPs (K ass ) were determined as described in Materials and Methods. Higher values of K ass reflect stronger binding. ( a) Binding to six low molecular mass Neu5Acα2-3Gal-containing SGPs differing by structure of penultimate sugar moieties. Wild type IAVs A/mallard/Alberta/279/1998 (H3N8) and A/ruddy turnstone/ Delaware/2378/1988 (H7N7) were tested in parallel with R7 and R2. Two to 4 experiments were performed on different days with one replicate for each virus-SGP pair per experiment. ( b) Binding to high molecular mass SGPs 3’SLN and 6’SLN. Two experiments were performed on different days with two replicates per each virus-SGP pair per experiment. All panels show the individual values adjusted for day as described in Materials and Methods with geometric mean (bars) and SDs. P values for the differences between Kass presented in the panel 3a are shown in S3 Table .
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