Additional file 5 of Facilitating population genomics of non-model organisms through optimized experimental design for reduced representation sequencing
Additional file 5. Reduced representation sequencing (RRS) laboratory protocol based on the protocol from Peterson et al. (2012) [15]. The protocol is scaled for use with 192 samples and with restriction enzymes EcoRI and MspI; the reagent volumes can be scaled down/up to suit other sample numbers;...
Main Authors: | , , , , , , , , , , , , , , , |
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Format: | Other Non-Article Part of Journal/Newspaper |
Language: | unknown |
Published: |
2021
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Subjects: | |
Online Access: | https://doi.org/10.6084/m9.figshare.16305507.v1 |
Summary: | Additional file 5. Reduced representation sequencing (RRS) laboratory protocol based on the protocol from Peterson et al. (2012) [15]. The protocol is scaled for use with 192 samples and with restriction enzymes EcoRI and MspI; the reagent volumes can be scaled down/up to suit other sample numbers; if other enzymes are used, the respective reaction conditions must be adjusted. |
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