Additional file 5 of Facilitating population genomics of non-model organisms through optimized experimental design for reduced representation sequencing

Additional file 5. Reduced representation sequencing (RRS) laboratory protocol based on the protocol from Peterson et al. (2012) [15]. The protocol is scaled for use with 192 samples and with restriction enzymes EcoRI and MspI; the reagent volumes can be scaled down/up to suit other sample numbers;...

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Bibliographic Details
Main Authors: Henrik Christiansen (5717978), Franz M. Heindler (5717981), Bart Hellemans (3246426), Quentin Jossart (532157), Francesca Pasotti (5665906), Henri Robert (6970418), Marie Verheye (11309976), Bruno Danis (811532), Marc Kochzius (242381), Frederik Leliaert (186222), Camille Moreau (811531), Tasnim Patel (11309979), Anton P. Van de Putte (11309982), Ann Vanreusel (220781), Filip A. M. Volckaert (8140614), Isa Schön (153646)
Format: Other Non-Article Part of Journal/Newspaper
Language:unknown
Published: 2021
Subjects:
Genetics
Biodiversity
Genome scan
Genotyping by sequencing
In silico digestion
RADseq
Southern Ocean
Online Access:https://doi.org/10.6084/m9.figshare.16305507.v1
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Summary:Additional file 5. Reduced representation sequencing (RRS) laboratory protocol based on the protocol from Peterson et al. (2012) [15]. The protocol is scaled for use with 192 samples and with restriction enzymes EcoRI and MspI; the reagent volumes can be scaled down/up to suit other sample numbers; if other enzymes are used, the respective reaction conditions must be adjusted.

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