Additional file 4 of Facilitating population genomics of non-model organisms through optimized experimental design for reduced representation sequencing

• Main Authors: Henrik Christiansen (5717978), Franz M. Heindler (5717981), Bart Hellemans (3246426), Quentin Jossart (532157), Francesca Pasotti (5665906), Henri Robert (6970418), Marie Verheye (11309976), Bruno Danis (811532), Marc Kochzius (242381), Frederik Leliaert (186222), Camille Moreau (811531), Tasnim Patel (11309979), Anton P. Van de Putte (11309982), Ann Vanreusel (220781), Filip A. M. Volckaert (8140614), Isa Schön (153646)
• Format: Other Non-Article Part of Journal/Newspaper
• Language: unknown
• Published: 2021
• Subjects: Genetics; Biodiversity; Genome scan; Genotyping by sequencing; In silico digestion; RADseq; Southern Ocean; Lowry
• Online Access: https://doi.org/10.6084/m9.figshare.16305504.v1

Additional file 4. Reduced representation sequencing (RRS) setups for seven individually optimized protocols. These setups were optimized in order to be run on a HiSeq 4000 platform (Illumina). The choice of restriction enzyme(s) and size window was optimized to obtain approximately 30× coverage (or half that value in a worst-case scenario) with the assumed genome size (conservatively estimated based on available information, see Table 2). Marker density was estimated as a comparable measure to the metastudy by Lowry et al. (2017) [34].