Additional file 4 of Facilitating population genomics of non-model organisms through optimized experimental design for reduced representation sequencing
Additional file 4. Reduced representation sequencing (RRS) setups for seven individually optimized protocols. These setups were optimized in order to be run on a HiSeq 4000 platform (Illumina). The choice of restriction enzyme(s) and size window was optimized to obtain approximately 30× coverage (or...
Main Authors: | , , , , , , , , , , , , , , , |
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Format: | Other Non-Article Part of Journal/Newspaper |
Language: | unknown |
Published: |
2021
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Subjects: | |
Online Access: | https://doi.org/10.6084/m9.figshare.16305504.v1 |
Summary: | Additional file 4. Reduced representation sequencing (RRS) setups for seven individually optimized protocols. These setups were optimized in order to be run on a HiSeq 4000 platform (Illumina). The choice of restriction enzyme(s) and size window was optimized to obtain approximately 30× coverage (or half that value in a worst-case scenario) with the assumed genome size (conservatively estimated based on available information, see Table 2). Marker density was estimated as a comparable measure to the metastudy by Lowry et al. (2017) [34]. |
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