Fig 2 -

• Main Authors: Takumi Tanaka (1977604), Ken Suzuki (75835), Hirokazu Ueda (2478403), Yuka Sameshima-Yamashita (6292238), Hiroko Kitamoto (6076307)
• Format: Still Image
• Language: unknown
• Published: 2021
• Subjects: Biochemistry; Microbiology; Biotechnology; Ecology; Science Policy; Environmental Sciences not elsewhere classified; Astronomical and Space Sciences not elsewhere classified; Biological Sciences not elsewhere classified; Chemical Sciences not elsewhere classified; basidiomycete yeast Pseudozyma anta.; ethanol precipitation steps; concentration; decreases ethanol use; PaE; ethanol treatment; Pseudozyma antarctica culture; sequential ammonium sulfate precipi.; Antarc*; Antarctica
• Online Access: https://doi.org/10.1371/journal.pone.0252811.g002

(a) Temporal changes in PaE activity in culture filtrates containing various amounts of ethanol. Ethanol was added to a final concentration of 0%, 10%, 25%, 50%, 75%, or 90% (v/v), and then the enzyme solution was stored at ambient temperature (RT) for up to 206 days. Relative activities are expressed as a percentage of the initial (0 day) activity of the control culture filtrate (containing 0% ethanol). Data are means ± standard deviation (SD) ( n = 3). (b) Residual PaE activity in the solution of partially purified PaE containing 20% ethanol. Partially purified PaE obtained by preparation with the combination of ammonium sulfate precipitation and ethanol precipitation was dissolved in 20% ethanol and stored at 4°C or RT for 230 days. Relative activities are expressed as a percentage of the initial (0 day) activity. Data are presented as the mean ± SD ( n = 3).