| Summary: | (A) Expression profile of genes encoding DNA methylation and demethylation-related enzymes during drought acclimation, growth, and response to dehydration stress. MET1/CMT3, DNA methyltransferase 1/chromomethylase 3; HDAC, histone deacetylase; DNMT, DNA methyltransferase; DRM2, domains rearranged methyltransferase 2; DDM1, decrease in DNA methylation 1; RDM4, RNA-directed DNA methylation 4; FDM1, factor of DNA methylation 1; DML/ROS1, DEMETER-like DNA demethylase/repressor of silencing 1. Scale bar represents the normalized expression of each gene between samples. Genes with significant expression changes (fold change ≥ 2 and adjusted p ≤ 0.05) in drought-acclimated plants compared with the non-acclimated control groups (A/F, A4/F4, and A18/F18) and in plants subjected to dehydration stress (SD/F, FD/F, A/F, F4D/F4, A4D/A4, F18D/F18, and A18D/A18) are indicated by asterisks (*) in the corresponding squares. (B) Global changes in the average proportion of methylated C, CG, CHH, and CHG. (C) Correlation analysis between the level of DNA methylation and the expression of methylation pathway genes. (D) Number statistic of DMRs between samples. Hypo- and hyper-DMRs are shown in the left and right columns, respectively.
|
|---|