Complementation of gene deletion in ΔPa PRO1 and ΔPa PRO2 mutants.

Secreted PaE in mutant strains (ΔPa PRO1 or ΔPa PRO2 ) bearing the plasmids pEmp-Pa PRO1 , pEmp-Pa PRO2 or pEmp, and cultured for 8 days, were detected by immunoblotting with anti-PaE antibody. PC, purified PaE as a positive control; black triangle, full-length PaE; gray triangles, degraded fragment...

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Main Authors: Natsuki Omae (10318504), Yuka Sameshima-Yamashita (6292238), Kazunori Ushimaru (3689119), Hideaki Koike (410323), Hiroko Kitamoto (6076307), Tomotake Morita (526538)
Format: Still Image
Language:unknown
Published: 2021
Subjects:
Biophysics
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Developmental Biology
Science Policy
Infectious Diseases
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
vacuolar protease function
yeast Pseudozyma antarctica
GB
plastic-degrading enzyme
48 h incubation
orthologous genes encoding proteases
3- L jar fermenter
XG 8
XG 8 Δ Pa
strain
XG 8 control
B orthologous genes
PaE
degradation fragments
Δ Pa
Antarc*
Antarctica
Online Access:https://doi.org/10.1371/journal.pone.0247462.g003
id ftsmithonian:oai:figshare.com:article/14231008
record_format openpolar
spelling ftsmithonian:oai:figshare.com:article/14231008 2023-05-15T13:43:17+02:00 Complementation of gene deletion in ΔPa PRO1 and ΔPa PRO2 mutants. Natsuki Omae (10318504) Yuka Sameshima-Yamashita (6292238) Kazunori Ushimaru (3689119) Hideaki Koike (410323) Hiroko Kitamoto (6076307) Tomotake Morita (526538) 2021-03-17T17:38:25Z https://doi.org/10.1371/journal.pone.0247462.g003 unknown https://figshare.com/articles/figure/Complementation_of_gene_deletion_in_Pa_i_PRO1_i_and_Pa_i_PRO2_i_mutants_/14231008 doi:10.1371/journal.pone.0247462.g003 CC BY 4.0 CC-BY Biophysics Biochemistry Microbiology Cell Biology Genetics Molecular Biology Developmental Biology Science Policy Infectious Diseases Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified vacuolar protease function yeast Pseudozyma antarctica GB plastic-degrading enzyme 48 h incubation orthologous genes encoding proteases 3- L jar fermenter XG 8 XG 8 Δ Pa strain XG 8 control B orthologous genes PaE degradation fragments Δ Pa Image Figure 2021 ftsmithonian https://doi.org/10.1371/journal.pone.0247462.g003 2021-03-23T16:29:03Z Secreted PaE in mutant strains (ΔPa PRO1 or ΔPa PRO2 ) bearing the plasmids pEmp-Pa PRO1 , pEmp-Pa PRO2 or pEmp, and cultured for 8 days, were detected by immunoblotting with anti-PaE antibody. PC, purified PaE as a positive control; black triangle, full-length PaE; gray triangles, degraded fragment of PaE in strain GB-4(0). Still Image Antarc* Antarctica Unknown
institution Open Polar
collection Unknown
op_collection_id ftsmithonian
language unknown
topic Biophysics
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Developmental Biology
Science Policy
Infectious Diseases
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
vacuolar protease function
yeast Pseudozyma antarctica
GB
plastic-degrading enzyme
48 h incubation
orthologous genes encoding proteases
3- L jar fermenter
XG 8
XG 8 Δ Pa
strain
XG 8 control
B orthologous genes
PaE
degradation fragments
Δ Pa
spellingShingle Biophysics
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Developmental Biology
Science Policy
Infectious Diseases
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
vacuolar protease function
yeast Pseudozyma antarctica
GB
plastic-degrading enzyme
48 h incubation
orthologous genes encoding proteases
3- L jar fermenter
XG 8
XG 8 Δ Pa
strain
XG 8 control
B orthologous genes
PaE
degradation fragments
Δ Pa
Natsuki Omae (10318504)
Yuka Sameshima-Yamashita (6292238)
Kazunori Ushimaru (3689119)
Hideaki Koike (410323)
Hiroko Kitamoto (6076307)
Tomotake Morita (526538)
Complementation of gene deletion in ΔPa PRO1 and ΔPa PRO2 mutants.
topic_facet Biophysics
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Developmental Biology
Science Policy
Infectious Diseases
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
vacuolar protease function
yeast Pseudozyma antarctica
GB
plastic-degrading enzyme
48 h incubation
orthologous genes encoding proteases
3- L jar fermenter
XG 8
XG 8 Δ Pa
strain
XG 8 control
B orthologous genes
PaE
degradation fragments
Δ Pa
description Secreted PaE in mutant strains (ΔPa PRO1 or ΔPa PRO2 ) bearing the plasmids pEmp-Pa PRO1 , pEmp-Pa PRO2 or pEmp, and cultured for 8 days, were detected by immunoblotting with anti-PaE antibody. PC, purified PaE as a positive control; black triangle, full-length PaE; gray triangles, degraded fragment of PaE in strain GB-4(0).
format Still Image
author Natsuki Omae (10318504)
Yuka Sameshima-Yamashita (6292238)
Kazunori Ushimaru (3689119)
Hideaki Koike (410323)
Hiroko Kitamoto (6076307)
Tomotake Morita (526538)
author_facet Natsuki Omae (10318504)
Yuka Sameshima-Yamashita (6292238)
Kazunori Ushimaru (3689119)
Hideaki Koike (410323)
Hiroko Kitamoto (6076307)
Tomotake Morita (526538)
author_sort Natsuki Omae (10318504)
title Complementation of gene deletion in ΔPa PRO1 and ΔPa PRO2 mutants.
title_short Complementation of gene deletion in ΔPa PRO1 and ΔPa PRO2 mutants.
title_full Complementation of gene deletion in ΔPa PRO1 and ΔPa PRO2 mutants.
title_fullStr Complementation of gene deletion in ΔPa PRO1 and ΔPa PRO2 mutants.
title_full_unstemmed Complementation of gene deletion in ΔPa PRO1 and ΔPa PRO2 mutants.
title_sort complementation of gene deletion in δpa pro1 and δpa pro2 mutants.
publishDate 2021
url https://doi.org/10.1371/journal.pone.0247462.g003
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_relation https://figshare.com/articles/figure/Complementation_of_gene_deletion_in_Pa_i_PRO1_i_and_Pa_i_PRO2_i_mutants_/14231008
doi:10.1371/journal.pone.0247462.g003
op_rights CC BY 4.0
op_rightsnorm CC-BY
op_doi https://doi.org/10.1371/journal.pone.0247462.g003
_version_ 1766186967594172416

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