Constructing deletion mutants of Pa PRO1 and Pa PRO2 .

Disruptive fragments for Pa PRO1 and Pa PRO2 are shown in (A) and (B), respectively. Numbers and arrows indicate primers. Descriptions of primers and amplicons are provided in S1 – S3 Tables. Deletion of the Pa PRO1 (C) or Pa PRO2 (D) gene was verified by PCR during positive and negative screening....

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Main Authors: Natsuki Omae (10318504), Yuka Sameshima-Yamashita (6292238), Kazunori Ushimaru (3689119), Hideaki Koike (410323), Hiroko Kitamoto (6076307), Tomotake Morita (526538)
Format: Still Image
Language:unknown
Published: 2021
Subjects:
Biophysics
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Developmental Biology
Science Policy
Infectious Diseases
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
vacuolar protease function
yeast Pseudozyma antarctica
GB
plastic-degrading enzyme
48 h incubation
orthologous genes encoding proteases
3- L jar fermenter
XG 8
XG 8 Δ Pa
strain
XG 8 control
B orthologous genes
PaE
degradation fragments
Δ Pa
Antarc*
Antarctica
Online Access:https://doi.org/10.1371/journal.pone.0247462.s004
id ftsmithonian:oai:figshare.com:article/14230960
record_format openpolar
spelling ftsmithonian:oai:figshare.com:article/14230960 2023-05-15T13:43:17+02:00 Constructing deletion mutants of Pa PRO1 and Pa PRO2 . Natsuki Omae (10318504) Yuka Sameshima-Yamashita (6292238) Kazunori Ushimaru (3689119) Hideaki Koike (410323) Hiroko Kitamoto (6076307) Tomotake Morita (526538) 2021-03-17T17:38:25Z https://doi.org/10.1371/journal.pone.0247462.s004 unknown https://figshare.com/articles/figure/Constructing_deletion_mutants_of_Pa_i_PRO1_i_and_Pa_i_PRO2_i_/14230960 doi:10.1371/journal.pone.0247462.s004 CC BY 4.0 CC-BY Biophysics Biochemistry Microbiology Cell Biology Genetics Molecular Biology Developmental Biology Science Policy Infectious Diseases Biological Sciences not elsewhere classified Chemical Sciences not elsewhere classified vacuolar protease function yeast Pseudozyma antarctica GB plastic-degrading enzyme 48 h incubation orthologous genes encoding proteases 3- L jar fermenter XG 8 XG 8 Δ Pa strain XG 8 control B orthologous genes PaE degradation fragments Δ Pa Image Figure 2021 ftsmithonian https://doi.org/10.1371/journal.pone.0247462.s004 2021-03-23T16:29:07Z Disruptive fragments for Pa PRO1 and Pa PRO2 are shown in (A) and (B), respectively. Numbers and arrows indicate primers. Descriptions of primers and amplicons are provided in S1 – S3 Tables. Deletion of the Pa PRO1 (C) or Pa PRO2 (D) gene was verified by PCR during positive and negative screening. (TIF) Still Image Antarc* Antarctica Unknown
institution Open Polar
collection Unknown
op_collection_id ftsmithonian
language unknown
topic Biophysics
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Developmental Biology
Science Policy
Infectious Diseases
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
vacuolar protease function
yeast Pseudozyma antarctica
GB
plastic-degrading enzyme
48 h incubation
orthologous genes encoding proteases
3- L jar fermenter
XG 8
XG 8 Δ Pa
strain
XG 8 control
B orthologous genes
PaE
degradation fragments
Δ Pa
spellingShingle Biophysics
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Developmental Biology
Science Policy
Infectious Diseases
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
vacuolar protease function
yeast Pseudozyma antarctica
GB
plastic-degrading enzyme
48 h incubation
orthologous genes encoding proteases
3- L jar fermenter
XG 8
XG 8 Δ Pa
strain
XG 8 control
B orthologous genes
PaE
degradation fragments
Δ Pa
Natsuki Omae (10318504)
Yuka Sameshima-Yamashita (6292238)
Kazunori Ushimaru (3689119)
Hideaki Koike (410323)
Hiroko Kitamoto (6076307)
Tomotake Morita (526538)
Constructing deletion mutants of Pa PRO1 and Pa PRO2 .
topic_facet Biophysics
Biochemistry
Microbiology
Cell Biology
Genetics
Molecular Biology
Developmental Biology
Science Policy
Infectious Diseases
Biological Sciences not elsewhere classified
Chemical Sciences not elsewhere classified
vacuolar protease function
yeast Pseudozyma antarctica
GB
plastic-degrading enzyme
48 h incubation
orthologous genes encoding proteases
3- L jar fermenter
XG 8
XG 8 Δ Pa
strain
XG 8 control
B orthologous genes
PaE
degradation fragments
Δ Pa
description Disruptive fragments for Pa PRO1 and Pa PRO2 are shown in (A) and (B), respectively. Numbers and arrows indicate primers. Descriptions of primers and amplicons are provided in S1 – S3 Tables. Deletion of the Pa PRO1 (C) or Pa PRO2 (D) gene was verified by PCR during positive and negative screening. (TIF)
format Still Image
author Natsuki Omae (10318504)
Yuka Sameshima-Yamashita (6292238)
Kazunori Ushimaru (3689119)
Hideaki Koike (410323)
Hiroko Kitamoto (6076307)
Tomotake Morita (526538)
author_facet Natsuki Omae (10318504)
Yuka Sameshima-Yamashita (6292238)
Kazunori Ushimaru (3689119)
Hideaki Koike (410323)
Hiroko Kitamoto (6076307)
Tomotake Morita (526538)
author_sort Natsuki Omae (10318504)
title Constructing deletion mutants of Pa PRO1 and Pa PRO2 .
title_short Constructing deletion mutants of Pa PRO1 and Pa PRO2 .
title_full Constructing deletion mutants of Pa PRO1 and Pa PRO2 .
title_fullStr Constructing deletion mutants of Pa PRO1 and Pa PRO2 .
title_full_unstemmed Constructing deletion mutants of Pa PRO1 and Pa PRO2 .
title_sort constructing deletion mutants of pa pro1 and pa pro2 .
publishDate 2021
url https://doi.org/10.1371/journal.pone.0247462.s004
genre Antarc*
Antarctica
genre_facet Antarc*
Antarctica
op_relation https://figshare.com/articles/figure/Constructing_deletion_mutants_of_Pa_i_PRO1_i_and_Pa_i_PRO2_i_/14230960
doi:10.1371/journal.pone.0247462.s004
op_rights CC BY 4.0
op_rightsnorm CC-BY
op_doi https://doi.org/10.1371/journal.pone.0247462.s004
_version_ 1766187006063280128

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