Constructing deletion mutants of Pa PRO1 and Pa PRO2 .
Disruptive fragments for Pa PRO1 and Pa PRO2 are shown in (A) and (B), respectively. Numbers and arrows indicate primers. Descriptions of primers and amplicons are provided in S1 – S3 Tables. Deletion of the Pa PRO1 (C) or Pa PRO2 (D) gene was verified by PCR during positive and negative screening....
Main Authors: | , , , , , |
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Format: | Still Image |
Language: | unknown |
Published: |
2021
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Subjects: | |
Online Access: | https://doi.org/10.1371/journal.pone.0247462.s004 |
Summary: | Disruptive fragments for Pa PRO1 and Pa PRO2 are shown in (A) and (B), respectively. Numbers and arrows indicate primers. Descriptions of primers and amplicons are provided in S1 – S3 Tables. Deletion of the Pa PRO1 (C) or Pa PRO2 (D) gene was verified by PCR during positive and negative screening. (TIF) |
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