| Summary: | The sustainable production of useful chemical building blocks, such as alcohols, remains one of the challenges of the 21st century, as processess still heavily rely on petroleum-based crude materials. The microbial production of these compounds from renewable resources offers an environmentally friendlier alternative. The study herein reports on the ability of mesophilic facultative anaerobes isolated from a geothermally-heated intertidal pool north of Húsavík, Iceland to produce 1,2-propanediol (1,2-PD) from methylpentoses. Majority of investigated strains belonged to genera Enterobacter, Shigella, and Escherichia. All 21 environmental isolates produced 1,2-PD from D-glucose, L-rhamnose, and L-fucose. During a preliminary study of the strains‘ abilities to synthesize 1,2-PD from these three model substrates, Enterobacter cloacae strains C1B, BB5C, Z1B, and BB1B and Escherichia fergusonii strain NT5CB yielded 1,2-PD titers 90% and higher that of theoretical. Assessment of the influence of initial concentration of L-rhamnose generally showed inhibition at loadings higher than 20 mM. Strictly anaerobic conditions were not required for the production of 1,2-PD as many strains showed highest titers when cultivated under microaerobic conditions. Unsurprisingly, lowest 1,2-PD yields were obtained at fully aerobic condition with the exception of Shigella sonnei (DSM 5570) that required the presence of oxygen for the successful synthesis of 1,2-PD. Sjálfbær framleiðsla á nytsamlegum efnasamböndum á borð við alkóhól er ein af mörgum áskorunum 21. aldar þar sem vinnsla þeirra veltur enn að miklu leyti á hráefnum úr jarðefnaeldsneyti. Framleiðsla þessara efnasambanda með örverum úr endurnýjanlegum orkugjöfum er mun umhverfisvænni kostur. Hér verður greint frá getu miðlungs hitakærra, valbundinna loftfælinna örvera sem einangraðar voru úr íslenskri sjávarfjöru við Húsavík til þess að framleiða 1,2-própandíól (1,2-PD) úr metýlpentósum. Meirihluti stofnanna tilheyrði ættkvíslunum Enterobacter, Shigella og Escherichia. Allir ...
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