Development of Nuclear Microsatellite Markers with Long (Tri-, Tetra-, Penta-, and Hexanucleotide) Motifs for Three Larch Species Based on the de novo Whole Genome Sequencing of Siberian Larch (Larix sibirica Ledeb.)

• Published in: Russian Journal of Genetics
• Main Authors: Oreshkova, N. V., Bondar, E. I., Putintseva, Yu. A., Sharov, V. V., Kuzmin, D. A., Krutovsky, K. V.
• Other Authors: Институт фундаментальной биологии и биотехнологии, Кафедра биофизики, Базовая кафедра защиты и современных технологии мониторинга лесов
• Format: Article in Journal/Newspaper
• Language: unknown
• Published: 2019
• Subjects: conifers; genetic diversity; heterozygosity; Larix; larch; microsatellite markers; SSR; whole-genome sequencing; 34.15.23; Sibirica
• Online Access: https://link.springer.com/article/10.1134/S1022795419040094; http://elib.sfu-kras.ru/handle/2311/129341; https://doi.org/10.1134/S1022795419040094

Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала. Siberian larch (Larix sibirica Ledeb.) is one of the major boreal tree species in Eurasia and has a considerable economic and ecological value. Despite that importance, the development and use of microsatellite markers in this species remain limited. Microsatellite markers are considered to be a valuable tool for estimation of population diversity and structure. Availability of a draft reference assembly of the Siberian larch genome allowed us to identify 1015 microsatellite loci or simple sequence repeats (SSRs) with tri-, tetra-, penta-, and hexanucleotide motifs. For 60 of them PCR primers were designed and tested for amplification in L. sibirica and for their within-genus transferability to L. gmelinii (Rupr.) Rupr. and L. cajanderi Mayr. Here, we present a set of 14 reliable and polymorphic new nuclear SSR markers that can be used for further population genetic studies, breeding programs, and timber origin identification.