Development of Nuclear Microsatellite Markers with Long (Tri-, Tetra-, Penta-, and Hexanucleotide) Motifs for Three Larch Species Based on the de novo Whole Genome Sequencing of Siberian Larch (Larix sibirica Ledeb.)
Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала. Siberian larch (Larix sibirica Ledeb.) is one of the major boreal tree species in Eurasia and has a considerable economic and ecological value. Despite that importance, the development and use of microsatellite marker...
Published in: | Russian Journal of Genetics |
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Online Access: | https://link.springer.com/article/10.1134/S1022795419040094 http://elib.sfu-kras.ru/handle/2311/129341 https://doi.org/10.1134/S1022795419040094 |
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ftsiberianfuniv:oai:elib.sfu-kras.ru:2311/129341 2023-05-15T18:19:41+02:00 Development of Nuclear Microsatellite Markers with Long (Tri-, Tetra-, Penta-, and Hexanucleotide) Motifs for Three Larch Species Based on the de novo Whole Genome Sequencing of Siberian Larch (Larix sibirica Ledeb.) Oreshkova, N. V. Bondar, E. I. Putintseva, Yu. A. Sharov, V. V. Kuzmin, D. A. Krutovsky, K. V. Институт фундаментальной биологии и биотехнологии Кафедра биофизики Базовая кафедра защиты и современных технологии мониторинга лесов 2019-04 https://link.springer.com/article/10.1134/S1022795419040094 http://elib.sfu-kras.ru/handle/2311/129341 https://doi.org/10.1134/S1022795419040094 unknown Russian Journal of Genetics Q4 Oreshkova, N. V. Development of Nuclear Microsatellite Markers with Long (Tri-, Tetra-, Penta-, and Hexanucleotide) Motifs for Three Larch Species Based on the de novo Whole Genome Sequencing of Siberian Larch (Larix sibirica Ledeb.) [Текст] / N. V. Oreshkova, E. I. Bondar, Yu. A. Putintseva, V. V. Sharov, D. A. Kuzmin, K. V. Krutovsky // Russian Journal of Genetics. — 2019. — Т. 55 (№ 4). — С. 444-450 10227954 https://link.springer.com/article/10.1134/S1022795419040094 http://elib.sfu-kras.ru/handle/2311/129341 doi:10.1134/S1022795419040094 conifers genetic diversity heterozygosity Larix larch microsatellite markers SSR whole-genome sequencing 34.15.23 Journal Article Published Journal Article 2019 ftsiberianfuniv https://doi.org/10.1134/S1022795419040094 2020-01-21T00:52:32Z Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала. Siberian larch (Larix sibirica Ledeb.) is one of the major boreal tree species in Eurasia and has a considerable economic and ecological value. Despite that importance, the development and use of microsatellite markers in this species remain limited. Microsatellite markers are considered to be a valuable tool for estimation of population diversity and structure. Availability of a draft reference assembly of the Siberian larch genome allowed us to identify 1015 microsatellite loci or simple sequence repeats (SSRs) with tri-, tetra-, penta-, and hexanucleotide motifs. For 60 of them PCR primers were designed and tested for amplification in L. sibirica and for their within-genus transferability to L. gmelinii (Rupr.) Rupr. and L. cajanderi Mayr. Here, we present a set of 14 reliable and polymorphic new nuclear SSR markers that can be used for further population genetic studies, breeding programs, and timber origin identification. Article in Journal/Newspaper Sibirica Siberian Federal University: Archiv Elektronnych SFU Russian Journal of Genetics 55 4 444 450 |
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Open Polar |
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Siberian Federal University: Archiv Elektronnych SFU |
op_collection_id |
ftsiberianfuniv |
language |
unknown |
topic |
conifers genetic diversity heterozygosity Larix larch microsatellite markers SSR whole-genome sequencing 34.15.23 |
spellingShingle |
conifers genetic diversity heterozygosity Larix larch microsatellite markers SSR whole-genome sequencing 34.15.23 Oreshkova, N. V. Bondar, E. I. Putintseva, Yu. A. Sharov, V. V. Kuzmin, D. A. Krutovsky, K. V. Development of Nuclear Microsatellite Markers with Long (Tri-, Tetra-, Penta-, and Hexanucleotide) Motifs for Three Larch Species Based on the de novo Whole Genome Sequencing of Siberian Larch (Larix sibirica Ledeb.) |
topic_facet |
conifers genetic diversity heterozygosity Larix larch microsatellite markers SSR whole-genome sequencing 34.15.23 |
description |
Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала. Siberian larch (Larix sibirica Ledeb.) is one of the major boreal tree species in Eurasia and has a considerable economic and ecological value. Despite that importance, the development and use of microsatellite markers in this species remain limited. Microsatellite markers are considered to be a valuable tool for estimation of population diversity and structure. Availability of a draft reference assembly of the Siberian larch genome allowed us to identify 1015 microsatellite loci or simple sequence repeats (SSRs) with tri-, tetra-, penta-, and hexanucleotide motifs. For 60 of them PCR primers were designed and tested for amplification in L. sibirica and for their within-genus transferability to L. gmelinii (Rupr.) Rupr. and L. cajanderi Mayr. Here, we present a set of 14 reliable and polymorphic new nuclear SSR markers that can be used for further population genetic studies, breeding programs, and timber origin identification. |
author2 |
Институт фундаментальной биологии и биотехнологии Кафедра биофизики Базовая кафедра защиты и современных технологии мониторинга лесов |
format |
Article in Journal/Newspaper |
author |
Oreshkova, N. V. Bondar, E. I. Putintseva, Yu. A. Sharov, V. V. Kuzmin, D. A. Krutovsky, K. V. |
author_facet |
Oreshkova, N. V. Bondar, E. I. Putintseva, Yu. A. Sharov, V. V. Kuzmin, D. A. Krutovsky, K. V. |
author_sort |
Oreshkova, N. V. |
title |
Development of Nuclear Microsatellite Markers with Long (Tri-, Tetra-, Penta-, and Hexanucleotide) Motifs for Three Larch Species Based on the de novo Whole Genome Sequencing of Siberian Larch (Larix sibirica Ledeb.) |
title_short |
Development of Nuclear Microsatellite Markers with Long (Tri-, Tetra-, Penta-, and Hexanucleotide) Motifs for Three Larch Species Based on the de novo Whole Genome Sequencing of Siberian Larch (Larix sibirica Ledeb.) |
title_full |
Development of Nuclear Microsatellite Markers with Long (Tri-, Tetra-, Penta-, and Hexanucleotide) Motifs for Three Larch Species Based on the de novo Whole Genome Sequencing of Siberian Larch (Larix sibirica Ledeb.) |
title_fullStr |
Development of Nuclear Microsatellite Markers with Long (Tri-, Tetra-, Penta-, and Hexanucleotide) Motifs for Three Larch Species Based on the de novo Whole Genome Sequencing of Siberian Larch (Larix sibirica Ledeb.) |
title_full_unstemmed |
Development of Nuclear Microsatellite Markers with Long (Tri-, Tetra-, Penta-, and Hexanucleotide) Motifs for Three Larch Species Based on the de novo Whole Genome Sequencing of Siberian Larch (Larix sibirica Ledeb.) |
title_sort |
development of nuclear microsatellite markers with long (tri-, tetra-, penta-, and hexanucleotide) motifs for three larch species based on the de novo whole genome sequencing of siberian larch (larix sibirica ledeb.) |
publishDate |
2019 |
url |
https://link.springer.com/article/10.1134/S1022795419040094 http://elib.sfu-kras.ru/handle/2311/129341 https://doi.org/10.1134/S1022795419040094 |
genre |
Sibirica |
genre_facet |
Sibirica |
op_relation |
Russian Journal of Genetics Q4 Oreshkova, N. V. Development of Nuclear Microsatellite Markers with Long (Tri-, Tetra-, Penta-, and Hexanucleotide) Motifs for Three Larch Species Based on the de novo Whole Genome Sequencing of Siberian Larch (Larix sibirica Ledeb.) [Текст] / N. V. Oreshkova, E. I. Bondar, Yu. A. Putintseva, V. V. Sharov, D. A. Kuzmin, K. V. Krutovsky // Russian Journal of Genetics. — 2019. — Т. 55 (№ 4). — С. 444-450 10227954 https://link.springer.com/article/10.1134/S1022795419040094 http://elib.sfu-kras.ru/handle/2311/129341 doi:10.1134/S1022795419040094 |
op_doi |
https://doi.org/10.1134/S1022795419040094 |
container_title |
Russian Journal of Genetics |
container_volume |
55 |
container_issue |
4 |
container_start_page |
444 |
op_container_end_page |
450 |
_version_ |
1766196879256715264 |