Development of Nuclear Microsatellite Markers with Long (Tri-, Tetra-, Penta-, and Hexanucleotide) Motifs for Three Larch Species Based on the de novo Whole Genome Sequencing of Siberian Larch (Larix sibirica Ledeb.)
Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала. Siberian larch (Larix sibirica Ledeb.) is one of the major boreal tree species in Eurasia and has a considerable economic and ecological value. Despite that importance, the development and use of microsatellite marker...
| Published in: | Russian Journal of Genetics |
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| Main Authors: | , , , , , |
| Other Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | unknown |
| Published: |
2019
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| Subjects: | |
| Online Access: | https://link.springer.com/article/10.1134/S1022795419040094 http://elib.sfu-kras.ru/handle/2311/129341 https://doi.org/10.1134/S1022795419040094 |
| Summary: | Текст статьи не публикуется в открытом доступе в соответствии с политикой журнала. Siberian larch (Larix sibirica Ledeb.) is one of the major boreal tree species in Eurasia and has a considerable economic and ecological value. Despite that importance, the development and use of microsatellite markers in this species remain limited. Microsatellite markers are considered to be a valuable tool for estimation of population diversity and structure. Availability of a draft reference assembly of the Siberian larch genome allowed us to identify 1015 microsatellite loci or simple sequence repeats (SSRs) with tri-, tetra-, penta-, and hexanucleotide motifs. For 60 of them PCR primers were designed and tested for amplification in L. sibirica and for their within-genus transferability to L. gmelinii (Rupr.) Rupr. and L. cajanderi Mayr. Here, we present a set of 14 reliable and polymorphic new nuclear SSR markers that can be used for further population genetic studies, breeding programs, and timber origin identification. |
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