Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against PathogenicVibrio coralliilyticusCausing Mortality of Pacific Oyster (Crassostrea gigas) Larvae
Vibrio coralliilyticusis one of the major pathogens causing mass mortality in marine bivalve larvae aquaculture. To prevent and controlVibriospp. infections in marine bivalve hatcheries, various antibiotics are overused, resulting in environmental pollution and the creation of multi-drug-resistant s...
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2020
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Online Access: | https://hdl.handle.net/10371/189741 https://doi.org/10.3390/microorganisms8060926 |
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ftseoulnuniv:oai:s-space.snu.ac.kr:10371/189741 2023-12-03T10:21:37+01:00 Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against PathogenicVibrio coralliilyticusCausing Mortality of Pacific Oyster (Crassostrea gigas) Larvae Kim, Hyoun Joong Giri, Sib Sankar Kim, Sang Guen Kim, Sang Wha Kwon, Jun Lee, Sung Bin Park, Se Chang Park, Se Chang 2020-07-22 https://hdl.handle.net/10371/189741 https://doi.org/10.3390/microorganisms8060926 영어 unknown MDPI AG Microorganisms, Vol.8 No.6, pp.926-10 2076-2607 https://hdl.handle.net/10371/189741 doi:10.3390/microorganisms8060926 000549262700001 2-s2.0-85087061747 108218 BACILLARY NECROSIS PSEUDOMONAS-PLECOGLOSSICIDA OSTREID HERPESVIRUS-1 PHAGE THERAPY INFECTION DISEASE FISH STRAINS TUBIASHII PATHOGENS Pacific oyster larvae mass mortality Vibrio coralliilyticus bacteriophage phage antibiotic substitution Article ART 2020 ftseoulnuniv https://doi.org/10.3390/microorganisms8060926 2023-11-03T01:38:12Z Vibrio coralliilyticusis one of the major pathogens causing mass mortality in marine bivalve larvae aquaculture. To prevent and controlVibriospp. infections in marine bivalve hatcheries, various antibiotics are overused, resulting in environmental pollution and the creation of multi-drug-resistant strains. Therefore, research on the development of antibiotic substitutes is required. In this study, we isolated two bacteriophages (phages) that specifically infected pathogenicV. coralliilyticusfrom an oyster hatchery and designated them as pVco-5 and pVco-7. Both phages were classified asPodoviridaeand were stable over a wide range of temperatures (4-37 degrees C) and at pH 7.0-9.0. Thus, both phages were suitable for application under the environmental conditions of an oyster hatchery. The two phages showed confirmed significant bactericidal efficacy against pathogenicV. coralliilyticusin an in vitro test. In the in vivo experiment, the phage pre-treated groups of Pacific oyster larvae showed significantly lower mortality againstV. coralliilyticusinfection than untreated control larvae. The results of the present study suggest that both phages could be used in the artificial marine bivalve seedling industry; not only to prevent pathogenicV. coralliilyticusinfection, but also to reduce antibiotic overuse. Y 1 Article in Journal/Newspaper Crassostrea gigas Pacific oyster Seoul National University: S-Space Pacific Microorganisms 8 6 926 |
institution |
Open Polar |
collection |
Seoul National University: S-Space |
op_collection_id |
ftseoulnuniv |
language |
unknown |
topic |
BACILLARY NECROSIS PSEUDOMONAS-PLECOGLOSSICIDA OSTREID HERPESVIRUS-1 PHAGE THERAPY INFECTION DISEASE FISH STRAINS TUBIASHII PATHOGENS Pacific oyster larvae mass mortality Vibrio coralliilyticus bacteriophage phage antibiotic substitution |
spellingShingle |
BACILLARY NECROSIS PSEUDOMONAS-PLECOGLOSSICIDA OSTREID HERPESVIRUS-1 PHAGE THERAPY INFECTION DISEASE FISH STRAINS TUBIASHII PATHOGENS Pacific oyster larvae mass mortality Vibrio coralliilyticus bacteriophage phage antibiotic substitution Kim, Hyoun Joong Giri, Sib Sankar Kim, Sang Guen Kim, Sang Wha Kwon, Jun Lee, Sung Bin Park, Se Chang Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against PathogenicVibrio coralliilyticusCausing Mortality of Pacific Oyster (Crassostrea gigas) Larvae |
topic_facet |
BACILLARY NECROSIS PSEUDOMONAS-PLECOGLOSSICIDA OSTREID HERPESVIRUS-1 PHAGE THERAPY INFECTION DISEASE FISH STRAINS TUBIASHII PATHOGENS Pacific oyster larvae mass mortality Vibrio coralliilyticus bacteriophage phage antibiotic substitution |
description |
Vibrio coralliilyticusis one of the major pathogens causing mass mortality in marine bivalve larvae aquaculture. To prevent and controlVibriospp. infections in marine bivalve hatcheries, various antibiotics are overused, resulting in environmental pollution and the creation of multi-drug-resistant strains. Therefore, research on the development of antibiotic substitutes is required. In this study, we isolated two bacteriophages (phages) that specifically infected pathogenicV. coralliilyticusfrom an oyster hatchery and designated them as pVco-5 and pVco-7. Both phages were classified asPodoviridaeand were stable over a wide range of temperatures (4-37 degrees C) and at pH 7.0-9.0. Thus, both phages were suitable for application under the environmental conditions of an oyster hatchery. The two phages showed confirmed significant bactericidal efficacy against pathogenicV. coralliilyticusin an in vitro test. In the in vivo experiment, the phage pre-treated groups of Pacific oyster larvae showed significantly lower mortality againstV. coralliilyticusinfection than untreated control larvae. The results of the present study suggest that both phages could be used in the artificial marine bivalve seedling industry; not only to prevent pathogenicV. coralliilyticusinfection, but also to reduce antibiotic overuse. Y 1 |
author2 |
Park, Se Chang |
format |
Article in Journal/Newspaper |
author |
Kim, Hyoun Joong Giri, Sib Sankar Kim, Sang Guen Kim, Sang Wha Kwon, Jun Lee, Sung Bin Park, Se Chang |
author_facet |
Kim, Hyoun Joong Giri, Sib Sankar Kim, Sang Guen Kim, Sang Wha Kwon, Jun Lee, Sung Bin Park, Se Chang |
author_sort |
Kim, Hyoun Joong |
title |
Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against PathogenicVibrio coralliilyticusCausing Mortality of Pacific Oyster (Crassostrea gigas) Larvae |
title_short |
Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against PathogenicVibrio coralliilyticusCausing Mortality of Pacific Oyster (Crassostrea gigas) Larvae |
title_full |
Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against PathogenicVibrio coralliilyticusCausing Mortality of Pacific Oyster (Crassostrea gigas) Larvae |
title_fullStr |
Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against PathogenicVibrio coralliilyticusCausing Mortality of Pacific Oyster (Crassostrea gigas) Larvae |
title_full_unstemmed |
Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against PathogenicVibrio coralliilyticusCausing Mortality of Pacific Oyster (Crassostrea gigas) Larvae |
title_sort |
isolation and characterization of two bacteriophages and their preventive effects against pathogenicvibrio coralliilyticuscausing mortality of pacific oyster (crassostrea gigas) larvae |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://hdl.handle.net/10371/189741 https://doi.org/10.3390/microorganisms8060926 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_relation |
Microorganisms, Vol.8 No.6, pp.926-10 2076-2607 https://hdl.handle.net/10371/189741 doi:10.3390/microorganisms8060926 000549262700001 2-s2.0-85087061747 108218 |
op_doi |
https://doi.org/10.3390/microorganisms8060926 |
container_title |
Microorganisms |
container_volume |
8 |
container_issue |
6 |
container_start_page |
926 |
_version_ |
1784269386221092864 |