Isolation and Characterization of Two Bacteriophages and Their Preventive Effects against PathogenicVibrio coralliilyticusCausing Mortality of Pacific Oyster (Crassostrea gigas) Larvae

Vibrio coralliilyticusis one of the major pathogens causing mass mortality in marine bivalve larvae aquaculture. To prevent and controlVibriospp. infections in marine bivalve hatcheries, various antibiotics are overused, resulting in environmental pollution and the creation of multi-drug-resistant s...

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Bibliographic Details
Published in:Microorganisms
Main Authors: Kim, Hyoun Joong, Giri, Sib Sankar, Kim, Sang Guen, Kim, Sang Wha, Kwon, Jun, Lee, Sung Bin, Park, Se Chang
Format: Article in Journal/Newspaper
Language:unknown
Published: MDPI AG 2020
Subjects:
BACILLARY NECROSIS
PSEUDOMONAS-PLECOGLOSSICIDA
OSTREID HERPESVIRUS-1
PHAGE THERAPY
INFECTION
DISEASE
FISH
STRAINS
TUBIASHII
PATHOGENS
Pacific oyster larvae
mass mortality
Vibrio coralliilyticus
bacteriophage
phage antibiotic substitution
Pacific
Crassostrea gigas
Pacific oyster
Online Access:https://hdl.handle.net/10371/189741
https://doi.org/10.3390/microorganisms8060926
Description
Summary:Vibrio coralliilyticusis one of the major pathogens causing mass mortality in marine bivalve larvae aquaculture. To prevent and controlVibriospp. infections in marine bivalve hatcheries, various antibiotics are overused, resulting in environmental pollution and the creation of multi-drug-resistant strains. Therefore, research on the development of antibiotic substitutes is required. In this study, we isolated two bacteriophages (phages) that specifically infected pathogenicV. coralliilyticusfrom an oyster hatchery and designated them as pVco-5 and pVco-7. Both phages were classified asPodoviridaeand were stable over a wide range of temperatures (4-37 degrees C) and at pH 7.0-9.0. Thus, both phages were suitable for application under the environmental conditions of an oyster hatchery. The two phages showed confirmed significant bactericidal efficacy against pathogenicV. coralliilyticusin an in vitro test. In the in vivo experiment, the phage pre-treated groups of Pacific oyster larvae showed significantly lower mortality againstV. coralliilyticusinfection than untreated control larvae. The results of the present study suggest that both phages could be used in the artificial marine bivalve seedling industry; not only to prevent pathogenicV. coralliilyticusinfection, but also to reduce antibiotic overuse. Y 1

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