Movie of L. mex Cas9 T7 ΔPF16 expressing an addback copy of PF16 from A CRISPR Cas9 high-throughput genome editing toolkit for kinetoplastids.

Videos of live promastigote cultures, recorded on an Olympus CKX41 microscope using a 20x LCACHN 0.4 NA objective for phase contrast and Hamamatsu ORCA-ER digital camera.

Bibliographic Details
Main Authors: Tom Beneke, Ross Madden, Laura Makin, Jessica Valli, Jack Sunter, Eva Gluenz
Format: Dataset
Language:unknown
Published: 2017
Subjects:
Microbiology
Leishmania
Trypanosoma
CRISPR
genome editing
T7 RNA polymerase
Olympus
Orca
Online Access:https://doi.org/10.6084/m9.figshare.4884377.v2
https://figshare.com/articles/media/Movie_of_L_mex_Cas9_T7_PF16_expressing_an_addback_copy_of_PF16_from_A_CRISPR_Cas9_high-throughput_genome_editing_toolkit_for_kinetoplastids/4884377
Description
Summary:Videos of live promastigote cultures, recorded on an Olympus CKX41 microscope using a 20x LCACHN 0.4 NA objective for phase contrast and Hamamatsu ORCA-ER digital camera.

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