Microsatellite markers for the common vole ( Microtus arvalis ) and their cross-species utility

Fragmentation of natural habitats of the common vole (Microtus arvalis) provides an excellent model system to study the consequences of restricted gene flow and small population sizes for isolated populations. Here we describe the isolation and characteristics of 10 autosomal and one X-linked micros...

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Bibliographic Details
Main Authors: Walser, Barbara, Heckel, Gerald
Language:English
Published: 2018
Subjects:
Online Access:http://doc.rero.ch/record/312096/files/10592_2007_Article_9355.pdf
Description
Summary:Fragmentation of natural habitats of the common vole (Microtus arvalis) provides an excellent model system to study the consequences of restricted gene flow and small population sizes for isolated populations. Here we describe the isolation and characteristics of 10 autosomal and one X-linked microsatellite marker. These new markers were tested in 24 voles from a natural population in eastern Germany. Loci were highly polymorphic with numbers of alleles per locus ranging from three to 26 and expected heterozygosities from 0.51 to 0.97. All loci except for the X-linked locus Mar105 followed Hardy-Weinberg expectations. Cross-species amplifications revealed that most loci were polymorphic as well in M.agrestis, M.thomasi, and M.pennsylvanicus