Entrapment in HydrIL gels: hydro-ionic liquid polymer gels for enzyme immobilization
Enzyme entrapment in hydro-ionic liquid gels (HydrIL gels) was explored as an enzyme immobilization strategy. The liquid phase of the gels was a mixture of buffer and tributyl methyl phosphonium alkanesulfonate [P 4441 ][RSO 3 ] ionic liquids. The ionic liquid modifies the liquid phase creating a mo...
Published in: | Catalysis Today |
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Main Authors: | , , , , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
2024
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Subjects: | |
Online Access: | https://pure.qub.ac.uk/en/publications/caeb2e8a-9ac9-494c-8c24-3b7e64b2edbf https://doi.org/10.1016/j.cattod.2024.114595 https://pureadmin.qub.ac.uk/ws/files/557108636/Gels.pdf |
Summary: | Enzyme entrapment in hydro-ionic liquid gels (HydrIL gels) was explored as an enzyme immobilization strategy. The liquid phase of the gels was a mixture of buffer and tributyl methyl phosphonium alkanesulfonate [P 4441 ][RSO 3 ] ionic liquids. The ionic liquid modifies the liquid phase creating a more “organic” hydrophobic microenvironment around the enzyme. In the esterification of a secondary alcohol by Candida antarctica Lipase B, a 20-fold enhancement in final substrate conversion was achieved using an HydrIL gel compared to a standard poly(acrylamide) hydrogel. Co-polymerization of the poly(acrylamide) matrix with acrylate monomers led to further increases in reaction rate. A CalB HydrIL gel was reused over 10 reaction cycles, achieving a consistent performance from the fifth cycle. IL leaching became undetectable from the third reaction cycle. |
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