Graphical visualization of general agreement between on-board light microscopy, qPCR, and chemical AZA quantification.

• Main Authors: Stephan Wietkamp (8997154), Bernd Krock (70720), Dave Clarke (3557780), Daniela Voß (8997156), Rafael Salas (643213), Jane Kilcoyne (1450081), Urban Tillmann (568612)
• Format: Still Image
• Language: unknown
• Published: 2020
• Subjects: Ecology; Marine Biology; Inorganic Chemistry; Environmental Sciences not elsewhere classified; Biological Sciences not elsewhere classified; Chemical Sciences not elsewhere classified; 1.2 x 10 5 cells L; azaspiracid-producing dinophyte species; North Sea waters; qPCR assays; North Atlantic; Amphidoma languida; AZA analogues; toxigenic species; 8.3 x 10 4 cells L; azaspiracid shellfish poisoning; 618 pg L; abundance; North Sea station; summer 2018 Representatives; AZP; toxigenic Amphidomataceae; North Sea; Azadinium poporum; Irish stations; marine dinophyte family Amphidomataceae; North Atlantic area
• Online Access: https://doi.org/10.1371/journal.pone.0235015.g006

Color code refers to the logarithmic scale (log 10 ). A–B: Abundances of amphidomatacean cells L -1 by (A) light microscopy and (B) qPCR estimations. C–D: Cells L -1 of Az . spinosum estimated by qPCR (C) and AZA quantities (pg L -1 ) of AZA-1, -2 and -33 (D) measured by LC-MS/MS. E–F: Cell numbers L -1 of Am . languida estimated by qPCR (E) and AZA quantities (pg L -1 ) of AZA-38 and AZA-39 (F) measured by LC-MS/MS.