Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria
A PCR approach was used to construct a database of nasA genes (called narB genes in cyanobacteria) and to detect the genetic potential for heterotrophic bacterial nitrate utilization in marine environments. A nasA-specific PCR primer set that could be used to selectively amplify the nasA gene from h...
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American Society for Microbiology
2001
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ftpubmed:oai:pubmedcentral.nih.gov:93313 2023-05-15T15:38:44+02:00 Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria Allen, Andrew E. Booth, Melissa G. Frischer, Marc E. Verity, Peter G. Zehr, Jonathan P. Zani, Sabino 2001-11 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93313 http://www.ncbi.nlm.nih.gov/pubmed/11679368 https://doi.org/10.1128/AEM.67.11.5343-5348.2001 en eng American Society for Microbiology http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93313 http://www.ncbi.nlm.nih.gov/pubmed/11679368 http://dx.doi.org/10.1128/AEM.67.11.5343-5348.2001 Copyright © 2001, American Society for Microbiology Microbial Ecology Text 2001 ftpubmed https://doi.org/10.1128/AEM.67.11.5343-5348.2001 2013-08-29T09:24:36Z A PCR approach was used to construct a database of nasA genes (called narB genes in cyanobacteria) and to detect the genetic potential for heterotrophic bacterial nitrate utilization in marine environments. A nasA-specific PCR primer set that could be used to selectively amplify the nasA gene from heterotrophic bacteria was designed. Using seawater DNA extracts obtained from microbial communities in the South Atlantic Bight, the Barents Sea, and the North Pacific Gyre, we PCR amplified and sequenced nasA genes. Our results indicate that several groups of heterotrophic bacterial nasA genes are common and widely distributed in oceanic environments. Text Barents Sea PubMed Central (PMC) Barents Sea Pacific Applied and Environmental Microbiology 67 11 5343 5348 |
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English |
topic |
Microbial Ecology |
spellingShingle |
Microbial Ecology Allen, Andrew E. Booth, Melissa G. Frischer, Marc E. Verity, Peter G. Zehr, Jonathan P. Zani, Sabino Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria |
topic_facet |
Microbial Ecology |
description |
A PCR approach was used to construct a database of nasA genes (called narB genes in cyanobacteria) and to detect the genetic potential for heterotrophic bacterial nitrate utilization in marine environments. A nasA-specific PCR primer set that could be used to selectively amplify the nasA gene from heterotrophic bacteria was designed. Using seawater DNA extracts obtained from microbial communities in the South Atlantic Bight, the Barents Sea, and the North Pacific Gyre, we PCR amplified and sequenced nasA genes. Our results indicate that several groups of heterotrophic bacterial nasA genes are common and widely distributed in oceanic environments. |
format |
Text |
author |
Allen, Andrew E. Booth, Melissa G. Frischer, Marc E. Verity, Peter G. Zehr, Jonathan P. Zani, Sabino |
author_facet |
Allen, Andrew E. Booth, Melissa G. Frischer, Marc E. Verity, Peter G. Zehr, Jonathan P. Zani, Sabino |
author_sort |
Allen, Andrew E. |
title |
Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria |
title_short |
Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria |
title_full |
Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria |
title_fullStr |
Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria |
title_full_unstemmed |
Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria |
title_sort |
diversity and detection of nitrate assimilation genes in marine bacteria |
publisher |
American Society for Microbiology |
publishDate |
2001 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93313 http://www.ncbi.nlm.nih.gov/pubmed/11679368 https://doi.org/10.1128/AEM.67.11.5343-5348.2001 |
geographic |
Barents Sea Pacific |
geographic_facet |
Barents Sea Pacific |
genre |
Barents Sea |
genre_facet |
Barents Sea |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93313 http://www.ncbi.nlm.nih.gov/pubmed/11679368 http://dx.doi.org/10.1128/AEM.67.11.5343-5348.2001 |
op_rights |
Copyright © 2001, American Society for Microbiology |
op_doi |
https://doi.org/10.1128/AEM.67.11.5343-5348.2001 |
container_title |
Applied and Environmental Microbiology |
container_volume |
67 |
container_issue |
11 |
container_start_page |
5343 |
op_container_end_page |
5348 |
_version_ |
1766370015803604992 |