Diversity and Detection of Nitrate Assimilation Genes in Marine Bacteria

A PCR approach was used to construct a database of nasA genes (called narB genes in cyanobacteria) and to detect the genetic potential for heterotrophic bacterial nitrate utilization in marine environments. A nasA-specific PCR primer set that could be used to selectively amplify the nasA gene from h...

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Bibliographic Details
Published in:Applied and Environmental Microbiology
Main Authors: Allen, Andrew E., Booth, Melissa G., Frischer, Marc E., Verity, Peter G., Zehr, Jonathan P., Zani, Sabino
Format: Text
Language:English
Published: American Society for Microbiology 2001
Subjects:
Microbial Ecology
Barents Sea
Pacific
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC93313
http://www.ncbi.nlm.nih.gov/pubmed/11679368
https://doi.org/10.1128/AEM.67.11.5343-5348.2001
Description
Summary:A PCR approach was used to construct a database of nasA genes (called narB genes in cyanobacteria) and to detect the genetic potential for heterotrophic bacterial nitrate utilization in marine environments. A nasA-specific PCR primer set that could be used to selectively amplify the nasA gene from heterotrophic bacteria was designed. Using seawater DNA extracts obtained from microbial communities in the South Atlantic Bight, the Barents Sea, and the North Pacific Gyre, we PCR amplified and sequenced nasA genes. Our results indicate that several groups of heterotrophic bacterial nasA genes are common and widely distributed in oceanic environments.

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