Lymphocyte subsets in Atlantic cod (Gadus morhua) interrogated by single-cell sequencing

Atlantic Cod (Gadus morhua) has lost the major histocompatibility complex class II presentation pathway. We recently identified CD8-positive T cells, B cells, and plasma cells in cod, but further characterisation of lymphocyte subsets is needed to elucidate immune adaptations triggered by the absenc...

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Bibliographic Details
Published in:Communications Biology
Main Authors: Guslund, Naomi Croft, Krabberød, Anders K., Nørstebø, Simen F., Solbakken, Monica Hongrø, Jakobsen, Kjetill S., Johansen, Finn-Eirik, Qiao, Shuo-Wang
Format: Text
Language:English
Published: Nature Publishing Group UK 2022
Subjects:
Article
atlantic cod
Gadus morhua
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC9276791/
http://www.ncbi.nlm.nih.gov/pubmed/35821077
https://doi.org/10.1038/s42003-022-03645-w
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Summary:Atlantic Cod (Gadus morhua) has lost the major histocompatibility complex class II presentation pathway. We recently identified CD8-positive T cells, B cells, and plasma cells in cod, but further characterisation of lymphocyte subsets is needed to elucidate immune adaptations triggered by the absence of CD4-positive T lymphocytes. Here, we use single-cell RNA sequencing to examine the lymphocyte heterogeneity in Atlantic cod spleen. We describe five T cell subsets and eight B cell subsets and propose a B cell trajectory of differentiation. Notably, we identify a subpopulation of T cells that are CD8-negative. Most of the CD8-negative T lymphocytes highly express the homologue of monocyte chemotactic protein 1b, and another subset of CD8-negative T lymphocytes express the homologue of the scavenger receptor m130. Uncovering the multiple lymphocyte cell sub-clusters reveals the different immune states present within the B and T cell populations, building a foundation for further work.

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