Parapoxvirus Papillomatosis in the Muskoxen (Ovibos moschatus): Genetical Differences between the Virus Causing New Outbreak in a Vaccinated Herd, the Vaccine Virus and a Local Orf Virus
Moens, U., I. Wold, S. D. Mathiesen, T. Jørgensen, D. Sørensen and T. Traavik: Parapoxvirus papillomatosis in the muskoxen (Ovibos moschatus): Genetical differences between the virus causing new outbreak in a vaccinated herd, the vaccine virus and a local orf virus. Acta vet. scand. 1990, 31, 17–25....
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ftpubmed:oai:pubmedcentral.nih.gov:8133252 2023-05-15T17:54:11+02:00 Parapoxvirus Papillomatosis in the Muskoxen (Ovibos moschatus): Genetical Differences between the Virus Causing New Outbreak in a Vaccinated Herd, the Vaccine Virus and a Local Orf Virus Moens, Ugo Wold, Inger Mathiesen, Svein D. Jorgensen, Trond Sørensen, Dag Traavik, Terje 1990-03-01 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133252/ http://www.ncbi.nlm.nih.gov/pubmed/2169188 https://doi.org/10.1186/BF03547573 en eng BioMed Central http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133252/ http://www.ncbi.nlm.nih.gov/pubmed/2169188 http://dx.doi.org/10.1186/BF03547573 © The Author(s) 1990 Acta Vet Scand Article Text 1990 ftpubmed https://doi.org/10.1186/BF03547573 2021-05-23T00:54:37Z Moens, U., I. Wold, S. D. Mathiesen, T. Jørgensen, D. Sørensen and T. Traavik: Parapoxvirus papillomatosis in the muskoxen (Ovibos moschatus): Genetical differences between the virus causing new outbreak in a vaccinated herd, the vaccine virus and a local orf virus. Acta vet. scand. 1990, 31, 17–25. – Since 1981 a domesticated muskoxen herd had been successfully vaccinated against papillomatosis with homogenated, glutaraldehyde inactivated papilloma tissue. In the fall of 1985 a new clinical outbreak of disease occurred, affecting previously infected as well as vaccinated animals. The purification of parapox virions directly from papilloma tissue and orf scabs collected in a local sheep farm was followed by restriction endonuclease analysis of viral DNA. The morphological identity of purified virus was controlled by electron microscopy. Comparison of restriction endonuclease digests (10 different enzymes) by gel electrophoresis demonstrated that the muskoxen parapoxvirus from the new outbreak 1985 differed considerably from the 2 other isolates (muskoxen 1981 and local orf). The latter viruses demonstrated a high degree of homology, but differences were evident after digestion with the enzyme EcoRI. During metrizamide gradient purification minor bands containing morphologically intact virions were isolated in addition to the major fractions. The restriction enzyme digests indicated that the virions of the minor bands differed from those in the major bands. Text ovibos moschatus PubMed Central (PMC) Acta Veterinaria Scandinavica 31 1 17 25 |
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Article Moens, Ugo Wold, Inger Mathiesen, Svein D. Jorgensen, Trond Sørensen, Dag Traavik, Terje Parapoxvirus Papillomatosis in the Muskoxen (Ovibos moschatus): Genetical Differences between the Virus Causing New Outbreak in a Vaccinated Herd, the Vaccine Virus and a Local Orf Virus |
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Article |
description |
Moens, U., I. Wold, S. D. Mathiesen, T. Jørgensen, D. Sørensen and T. Traavik: Parapoxvirus papillomatosis in the muskoxen (Ovibos moschatus): Genetical differences between the virus causing new outbreak in a vaccinated herd, the vaccine virus and a local orf virus. Acta vet. scand. 1990, 31, 17–25. – Since 1981 a domesticated muskoxen herd had been successfully vaccinated against papillomatosis with homogenated, glutaraldehyde inactivated papilloma tissue. In the fall of 1985 a new clinical outbreak of disease occurred, affecting previously infected as well as vaccinated animals. The purification of parapox virions directly from papilloma tissue and orf scabs collected in a local sheep farm was followed by restriction endonuclease analysis of viral DNA. The morphological identity of purified virus was controlled by electron microscopy. Comparison of restriction endonuclease digests (10 different enzymes) by gel electrophoresis demonstrated that the muskoxen parapoxvirus from the new outbreak 1985 differed considerably from the 2 other isolates (muskoxen 1981 and local orf). The latter viruses demonstrated a high degree of homology, but differences were evident after digestion with the enzyme EcoRI. During metrizamide gradient purification minor bands containing morphologically intact virions were isolated in addition to the major fractions. The restriction enzyme digests indicated that the virions of the minor bands differed from those in the major bands. |
format |
Text |
author |
Moens, Ugo Wold, Inger Mathiesen, Svein D. Jorgensen, Trond Sørensen, Dag Traavik, Terje |
author_facet |
Moens, Ugo Wold, Inger Mathiesen, Svein D. Jorgensen, Trond Sørensen, Dag Traavik, Terje |
author_sort |
Moens, Ugo |
title |
Parapoxvirus Papillomatosis in the Muskoxen (Ovibos moschatus): Genetical Differences between the Virus Causing New Outbreak in a Vaccinated Herd, the Vaccine Virus and a Local Orf Virus |
title_short |
Parapoxvirus Papillomatosis in the Muskoxen (Ovibos moschatus): Genetical Differences between the Virus Causing New Outbreak in a Vaccinated Herd, the Vaccine Virus and a Local Orf Virus |
title_full |
Parapoxvirus Papillomatosis in the Muskoxen (Ovibos moschatus): Genetical Differences between the Virus Causing New Outbreak in a Vaccinated Herd, the Vaccine Virus and a Local Orf Virus |
title_fullStr |
Parapoxvirus Papillomatosis in the Muskoxen (Ovibos moschatus): Genetical Differences between the Virus Causing New Outbreak in a Vaccinated Herd, the Vaccine Virus and a Local Orf Virus |
title_full_unstemmed |
Parapoxvirus Papillomatosis in the Muskoxen (Ovibos moschatus): Genetical Differences between the Virus Causing New Outbreak in a Vaccinated Herd, the Vaccine Virus and a Local Orf Virus |
title_sort |
parapoxvirus papillomatosis in the muskoxen (ovibos moschatus): genetical differences between the virus causing new outbreak in a vaccinated herd, the vaccine virus and a local orf virus |
publisher |
BioMed Central |
publishDate |
1990 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133252/ http://www.ncbi.nlm.nih.gov/pubmed/2169188 https://doi.org/10.1186/BF03547573 |
genre |
ovibos moschatus |
genre_facet |
ovibos moschatus |
op_source |
Acta Vet Scand |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133252/ http://www.ncbi.nlm.nih.gov/pubmed/2169188 http://dx.doi.org/10.1186/BF03547573 |
op_rights |
© The Author(s) 1990 |
op_doi |
https://doi.org/10.1186/BF03547573 |
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Acta Veterinaria Scandinavica |
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31 |
container_issue |
1 |
container_start_page |
17 |
op_container_end_page |
25 |
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1766161912927617024 |