Parapoxvirus Papillomatosis in the Muskoxen (Ovibos moschatus): Genetical Differences between the Virus Causing New Outbreak in a Vaccinated Herd, the Vaccine Virus and a Local Orf Virus
Moens, U., I. Wold, S. D. Mathiesen, T. Jørgensen, D. Sørensen and T. Traavik: Parapoxvirus papillomatosis in the muskoxen (Ovibos moschatus): Genetical differences between the virus causing new outbreak in a vaccinated herd, the vaccine virus and a local orf virus. Acta vet. scand. 1990, 31, 17–25....
| Published in: | Acta Veterinaria Scandinavica |
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| Main Authors: | , , , , , |
| Format: | Text |
| Language: | English |
| Published: |
BioMed Central
1990
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| Subjects: | |
| Online Access: | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC8133252/ http://www.ncbi.nlm.nih.gov/pubmed/2169188 https://doi.org/10.1186/BF03547573 |
| Summary: | Moens, U., I. Wold, S. D. Mathiesen, T. Jørgensen, D. Sørensen and T. Traavik: Parapoxvirus papillomatosis in the muskoxen (Ovibos moschatus): Genetical differences between the virus causing new outbreak in a vaccinated herd, the vaccine virus and a local orf virus. Acta vet. scand. 1990, 31, 17–25. – Since 1981 a domesticated muskoxen herd had been successfully vaccinated against papillomatosis with homogenated, glutaraldehyde inactivated papilloma tissue. In the fall of 1985 a new clinical outbreak of disease occurred, affecting previously infected as well as vaccinated animals. The purification of parapox virions directly from papilloma tissue and orf scabs collected in a local sheep farm was followed by restriction endonuclease analysis of viral DNA. The morphological identity of purified virus was controlled by electron microscopy. Comparison of restriction endonuclease digests (10 different enzymes) by gel electrophoresis demonstrated that the muskoxen parapoxvirus from the new outbreak 1985 differed considerably from the 2 other isolates (muskoxen 1981 and local orf). The latter viruses demonstrated a high degree of homology, but differences were evident after digestion with the enzyme EcoRI. During metrizamide gradient purification minor bands containing morphologically intact virions were isolated in addition to the major fractions. The restriction enzyme digests indicated that the virions of the minor bands differed from those in the major bands. |
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