Development and characterization of thirteen novel microsatellite markers for use in Greenland sharks (Somniosus microcephalus), with cross-amplification in Pacific sleeper sharks (Somniosus pacificus)

OBJECTIVE: The objectives of this work are to isolate, develop, and characterize polymorphic microsatellite markers for use in Greenland sharks (Somniosus microcephalus). RESULTS: Thirteen microsatellite loci were successfully amplified and yielded multi-locus genotypes for 36 S. microcephalus indiv...

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Bibliographic Details
Published in:BMC Research Notes
Main Authors: Swintek, Meaghan A., Walter, Ryan P.
Format: Text
Language:English
Published: BioMed Central 2021
Subjects:
Research Note
Greenland
Pacific
Svalbard
Somniosus microcephalus
Online Access:http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7816355/
https://doi.org/10.1186/s13104-021-05447-5
Description
Summary:OBJECTIVE: The objectives of this work are to isolate, develop, and characterize polymorphic microsatellite markers for use in Greenland sharks (Somniosus microcephalus). RESULTS: Thirteen microsatellite loci were successfully amplified and yielded multi-locus genotypes for 36 S. microcephalus individuals from Grise Fjord (n = 16) and Svalbard (n = 20). Each locus yielded between 2 and 9 alleles and observed heterozygosity ranged from 0.11 to 0.70 when estimated across both sites. One locus and three loci deviated from HWE following Bonferroni correction, for individuals sampled from Grise Fjord and Svalbard, respectively. Cross-amplification was successful at every locus for five of the ten S. pacificus individuals.

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