An ice-binding protein from an Arctic grass, Leymus mollis
Several cold-hardy grasses have been shown to have ice-binding proteins (IBPs) that protect against freeze-thaw injury. Here, we looked for IBP activity in an Alaskan coastal grass that had not previously been examined, Leymus mollis (Pooidae). Rhizome tissue had strong ice-structuring and ice recry...
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ftpubmed:oai:pubmedcentral.nih.gov:7383708 2023-05-15T15:01:21+02:00 An ice-binding protein from an Arctic grass, Leymus mollis Sformo, Todd L. Raymond, James A. 2020-06-26 http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383708/ https://doi.org/10.12688/f1000research.24328.1 en eng F1000 Research Limited http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383708/ http://dx.doi.org/10.12688/f1000research.24328.1 Copyright: © 2020 Sformo TL and Raymond JA http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. CC-BY F1000Res Brief Report Text 2020 ftpubmed https://doi.org/10.12688/f1000research.24328.1 2020-08-09T00:30:02Z Several cold-hardy grasses have been shown to have ice-binding proteins (IBPs) that protect against freeze-thaw injury. Here, we looked for IBP activity in an Alaskan coastal grass that had not previously been examined, Leymus mollis (Pooidae). Rhizome tissue had strong ice-structuring and ice recrystallization inhibiting (IRI) activities, indicating the probable presence of IBPs. The gene sequence of an IBP was obtained. The sequence encoded a 118-amino acid IRI domain that contained eight repeats. A 3D structure of the IRI domain was predicted from the structure of the IRI domain of the perennial ryegrass Lolium perenne. The predicted structure appeared to have the same eight beta-roll coils found in the L. perenne IBP. Text Arctic PubMed Central (PMC) Arctic F1000Research 9 648 |
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Open Polar |
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PubMed Central (PMC) |
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ftpubmed |
language |
English |
topic |
Brief Report |
spellingShingle |
Brief Report Sformo, Todd L. Raymond, James A. An ice-binding protein from an Arctic grass, Leymus mollis |
topic_facet |
Brief Report |
description |
Several cold-hardy grasses have been shown to have ice-binding proteins (IBPs) that protect against freeze-thaw injury. Here, we looked for IBP activity in an Alaskan coastal grass that had not previously been examined, Leymus mollis (Pooidae). Rhizome tissue had strong ice-structuring and ice recrystallization inhibiting (IRI) activities, indicating the probable presence of IBPs. The gene sequence of an IBP was obtained. The sequence encoded a 118-amino acid IRI domain that contained eight repeats. A 3D structure of the IRI domain was predicted from the structure of the IRI domain of the perennial ryegrass Lolium perenne. The predicted structure appeared to have the same eight beta-roll coils found in the L. perenne IBP. |
format |
Text |
author |
Sformo, Todd L. Raymond, James A. |
author_facet |
Sformo, Todd L. Raymond, James A. |
author_sort |
Sformo, Todd L. |
title |
An ice-binding protein from an Arctic grass, Leymus mollis |
title_short |
An ice-binding protein from an Arctic grass, Leymus mollis |
title_full |
An ice-binding protein from an Arctic grass, Leymus mollis |
title_fullStr |
An ice-binding protein from an Arctic grass, Leymus mollis |
title_full_unstemmed |
An ice-binding protein from an Arctic grass, Leymus mollis |
title_sort |
ice-binding protein from an arctic grass, leymus mollis |
publisher |
F1000 Research Limited |
publishDate |
2020 |
url |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383708/ https://doi.org/10.12688/f1000research.24328.1 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
F1000Res |
op_relation |
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7383708/ http://dx.doi.org/10.12688/f1000research.24328.1 |
op_rights |
Copyright: © 2020 Sformo TL and Raymond JA http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
op_rightsnorm |
CC-BY |
op_doi |
https://doi.org/10.12688/f1000research.24328.1 |
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F1000Research |
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9 |
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648 |
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